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AB129495

Anti-PHF16 antibody

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(1 Publication)

Rabbit Polyclonal PHF16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human JADE3 aa 750 to C-terminus.

View Alternative Names

KIAA0215, PHF16, JADE3, Protein Jade-3, Jade family PHD finger protein 3, PHD finger protein 16

2 Images
Immunoprecipitation - Anti-PHF16 antibody (AB129495)
  • IP

Unknown

Immunoprecipitation - Anti-PHF16 antibody (AB129495)

Detection of PHF16 by Western Blot of Immunprecipitate.
ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : control IgG.
Predicted band size : 94 kDa.
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PHF16 antibody (ab129495)

Predicted band size: 94 kDa

false

Western blot - Anti-PHF16 antibody (AB129495)
  • WB

Unknown

Western blot - Anti-PHF16 antibody (AB129495)

All lanes:

Western blot - Anti-PHF16 antibody (ab129495) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 94 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human JADE3 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHF16 also known as JADE3 is a protein with a mass of approximately 90 kDa. It is a member of the plant homeodomain finger family of proteins. PHF16 contains domains that mediate interactions with other proteins indicating its role in chromatin remodeling and transcription regulation. This protein is widely expressed in tissues including brain liver and lung. Its localization in these tissues suggests its involvement in various cellular processes.
Biological function summary

PHF16 interacts with a range of molecular complexes related to gene expression regulation. It is a component of the HBO1 complex which plays a role in histone acetylation. This complex modifies chromatin structure to facilitate access to DNA for transcription machinery. PHF16 acts by binding to specific histone marks participating in the regulation of transcriptional activation of genes. These functions make it an important player in maintaining proper gene expression levels across diverse cell types.

Pathways

PHF16 influences transcriptional regulation pathways and epigenetic modification pathways. Within these frameworks it closely interacts with other proteins like HBO1 and ING5. PHF16 contributes to the modification of histone acetylation patterns which is important for transcriptional regulation. It assists in setting the epigenetic landscape that determines the transcriptional output of genes involved in cell cycle and development.

Altered expression or mutation of PHF16 links to several pathological conditions including cancer and developmental disorders. For example dysregulation of PHF16 expression may contribute to tumorigenesis particularly in breast cancer due to its role in regulating genes essential for cell proliferation. Moreover interactions with proteins like HBO1 and ING5 highlight its importance in pathways disrupted in developmental diseases where epigenetic misregulation plays a significant role. Understanding PHF16's function and interactions provides critical insights into its potential as a therapeutic target in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity.
See full target information JADE3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:8157 PubMed28811661

2017

Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Pardo,Lu Yu,Shihpei Shen,Peri Tate,Daniel Bode,Blake L Letney,Dawn E Quelle,William Skarnes,Jyoti S Choudhary
View all publications
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