AB211365

Anti-PHGDH/Malate dehydrogenase antibody

Name: Anti-PHGDH/Malate dehydrogenase antibody (ab211365) | Abcam
Brand: Abcam Limited
SKU: AB211365
Price: 645 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PHGDH/Malate dehydrogenase antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHGDH aa 150-350.

View Alternative Names

PGDH3, PHGDH, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 2-oxoglutarate reductase, Malate dehydrogenase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling PHGDH/Malate dehydrogenase with ab211365 antibody at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunofluorescent analysis of U 2 OS cells cells (PFA-fixed/Triton X-100 permeabilized) labeling PHGDH/Malate dehydrogenase with ab211365 at 4 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling PHGDH/Malate dehydrogenase with ab211365 antibody at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemical analysis of paraffin embedded human breast tissue labeling PHGDH/Malate dehydrogenase with ab211365 antibody at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemical analysis of paraffin embedded human skin tissue labeling PHGDH/Malate dehydrogenase with ab211365 antibody at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

All lanes:

Western blot - Anti-PHGDH/Malate dehydrogenase antibody (ab211365) at 0.4 µg/mL

Lane 1:

SK-MEL-30 (Human cell line) cell lysate

Lane 2:

A549 (Human lung carcinoma cell line) cell lysate

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human PHGDH aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

O43175

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate dehydrogenase also known as PHGDH is an enzyme that catalyzes the conversion of 3-phosphoglycerate to 3-phosphohydroxypyruvate. It has an approximate mass of 57 kDa. The PHGDH is located in the cytoplasm and mitochondria where it plays a role in serine biosynthesis. Malate dehydrogenase might also get mentioned together with PHGDH because both enzymes link to metabolic processes. The expression of PHGDH is noted in various tissues with higher levels observed in some cancer cells.

Biological function summary

PHGDH is essential for the serine synthesis pathway and cellular metabolism regulation. It does not form part of large complexes but operates independently to convert substrates necessary for cell growth and proliferation. The enzyme supports the synthesis of nucleotides and proteins impacting cell cycle and survival. Researchers investigate PHGDH to understand its role in metabolic reprogramming especially in cancer cells.

Pathways

PHGDH is important in the serine biosynthesis pathway which is part of the larger glycolytic process. It connects to pathways that contribute to cellular carbon metabolism and energy production. PHGDH interacts with glutamate dehydrogenase protein (GDH) and also influences one-carbon metabolism and redox balance within the cell. These pathways play important roles in providing building blocks for nucleotide and lipid biosynthesis.

PHGDH has relevance in cancer and neurological disorders. Alterations and overexpression of PHGDH have been linked to oncogenesis and tumor growth particularly in breast cancer. The enzyme also associates with neurodegenerative diseases as proper serine levels are important for neuron function. In both conditions PHGDH becomes connected with glutamate dehydrogenase as alterations can impact amino acid metabolism and neurotransmitter balance. The study of PHGDH inhibitors is gaining attention for therapeutic potentials in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

See full target information PHGDH

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 58:e13755 PubMed39329203

2024

Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexiang Zhao,Kang Tang,Gurong Jiang,Xinyan Yang,Manman Cui,Cong Wan,Zhaoxiang Ouyang,Yi Zheng,Zhaoting Liu,Mei Wang,Xiao-Yang Zhao,Gang Chang

Nature metabolism 1:158-171 PubMed31106291

2019

Mitochondrial complex III is necessary for endothelial cell proliferation during angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren P Diebold,Hyea Jin Gil,Peng Gao,Carlos A Martinez,Samuel E Weinberg,Navdeep S Chandel

Cancer research 78:1972-1985 PubMed29654155

2018

ME1 Regulates NADPH Homeostasis to Promote Gastric Cancer Growth and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Xin Lu,Huai-Qiang Ju,Ze-Xian Liu,Dong-Liang Chen,Yun Wang,Qi Zhao,Qi-Nian Wu,Zhao-Lei Zeng,Hai-Bo Qiu,Pei-Shan Hu,Zhi-Qiang Wang,Dong-Sheng Zhang,Feng Wang,Rui-Hua Xu

Biochemical pharmacology 151:245-251 PubMed29305854

2018

Astrocytes in primary cultures express serine racemase, synthesize d-serine and acquire A1 reactive astrocyte features.

Applications

Unspecified application

Species

Unspecified reactive species

Suyan Li,Yota Uno,Uwe Rudolph,Johanna Cobb,Jing Liu,Thea Anderson,Deborah Levy,Darrick T Balu,Joseph T Coyle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHGDH/Malate dehydrogenase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunocytochemistry - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Western blot - Anti-PHGDH/Malate dehydrogenase antibody (AB211365)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise