AB86244

Anti-PHIP antibody

Name: Anti-PHIP antibody (ab86244) | Abcam
Brand: Abcam Limited
SKU: AB86244

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal PHIP antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PHIP aa 1600-1650.

View Alternative Names

DCAF14, WDR11, PHIP, PH-interacting protein, DDB1- and CUL4-associated factor 14, IRS-1 PH domain-binding protein, WD repeat-containing protein 11

1 Images

Unknown

Western blot - Anti-PHIP antibody (AB86244)

All lanes:

Western blot - Anti-PHIP antibody (ab86244) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 207 kDa

Observed band size: 230 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PHIP aa 1600-1650. The exact immunogen used to generate this antibody is proprietary information.

Q8WWQ0

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHIP also known as pleckstrin homology domain interacting protein is an adapter protein that interacts with insulin receptor substrates. It has a mass of approximately 100 kDa. PHIP is expressed in various tissues including the brain and liver. The protein is involved in intracellular signaling pathways by binding phosphoinositides which are important for cellular communication and growth regulation.

Biological function summary

PHIP plays a significant role in cell proliferation and survival. It contributes to the regulation of the cell cycle by influencing transcription factors and other core components involved in cell growth. PHIP is not only a standalone protein but also part of complexes that modulate chromatin affecting gene expression patterns.

Pathways

PHIP is integrated into insulin signaling and PI3K/AKT pathways. In these pathways PHIP interacts with key proteins like AKT1 supporting cellular responses to insulin and growth factors. The involvement of PHIP in these pathways highlights its role in metabolic regulation and its impact on cell growth and metabolism.

PHIP has links to cancer and diabetes. Its dysregulation contributes to abnormal cell proliferation often observed in cancers. PHIP's interaction with insulin signaling components such as IRS1 demonstrates its relevance to diabetes management. These connections show PHIP's potential as a therapeutic target for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti-apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization.

See full target information PHIP

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27487 PubMed39523440

2024

RepID as a potential biomarker and therapeutic target for lung neuroendocrine tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Uk Park,Jae-Hyun Jo,Sangjune Kim,Christophe E Redon,Mirit I Aladjem,Yuri Seo,Se Jin Jang,Sang-Min Jang

Cell communication and signaling : CCS 21:219 PubMed37612584

2023

RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyun Jo,Jong-Uk Park,Yeong-Mu Kim,Seon-Mi Ok,Dong-Kyu Kim,Dong-Hyun Jung,Hye-Ji Kim,Hyun-A Seong,Hyo Je Cho,Jihoon Nah,Sangjune Kim,Haiqing Fu,Christophe E Redon,Mirit I Aladjem,Sang-Min Jang

Cell reports 34:108669 PubMed33503431

2021

DCAF14 promotes stalled fork stability to maintain genome integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Arik Townsend,Gabriella Lora,Justin Engel,Neysha Tirado-Class,Huzefa Dungrawala

Molecular therapy. Nucleic acids 23:185-199 PubMed33376626

2020

circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Su,Yuehong Shen,Yufan Wang,Feng Wang,Xia Hong,Yuling Chen,Yuntao Lin,Hongyu Yang

Cell metabolism 31:1107-1119.e12 PubMed32492392

2020

Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Gaëlle Marenne,Audrey E Hendricks,Aliki Perdikari,Rebecca Bounds,Felicity Payne,Julia M Keogh,Christopher J Lelliott,Elana Henning,Saad Pathan,Sofie Ashford,Elena G Bochukova,Vanisha Mistry,Allan Daly,Caroline Hayward,Nicholas J Wareham,Stephen O'Rahilly,Claudia Langenberg,Eleanor Wheeler,Eleftheria Zeggini,I Sadaf Farooqi,Inês Barroso

PloS one 8:e83190 PubMed24391744

2013

RVX-208, an inducer of ApoA-I in humans, is a BET bromodomain antagonist.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin G McLure,Emily M Gesner,Laura Tsujikawa,Olesya A Kharenko,Sarah Attwell,Eric Campeau,Sylwia Wasiak,Adam Stein,Andre White,Eric Fontano,Robert K Suto,Norman C W Wong,Gregory S Wagner,Henrik C Hansen,Peter R Young

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHIP antibody

Western blot - Anti-PHIP antibody (AB86244)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise