Rabbit Polyclonal PHIP antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PHIP aa 1600-1650.
Images
Publications
Filter by
- Cell communication and signaling : CCS 21:2192023RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae-Hyun Jo,Jong-Uk Park,Yeong-Mu Kim,Seon-Mi Ok,Dong-Kyu Kim,Dong-Hyun Jung,Hye-Ji Kim,Hyun-A Seong,Hyo Je Cho,Jihoon Nah,Sangjune Kim,Haiqing Fu,Christophe E Redon,Mirit I Aladjem,Sang-Min JangPubMed 37612584
- Cell reports 34:1086692021DCAF14 promotes stalled fork stability to maintain genome integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArik Townsend,Gabriella Lora,Justin Engel,Neysha Tirado-Class,Huzefa DungrawalaPubMed 33503431
- Molecular therapy. Nucleic acids 23:185-1992020circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Su,Yuehong Shen,Yufan Wang,Feng Wang,Xia Hong,Yuling Chen,Yuntao Lin,Hongyu YangPubMed 33376626
- Cell metabolism 31:1107-1119.e122020Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaëlle Marenne,Audrey E Hendricks,Aliki Perdikari,Rebecca Bounds,Felicity Payne,Julia M Keogh,Christopher J Lelliott,Elana Henning,Saad Pathan,Sofie Ashford,Elena G Bochukova,Vanisha Mistry,Allan Daly,Caroline Hayward,Nicholas J Wareham,Stephen O'Rahilly,Claudia Langenberg,Eleanor Wheeler,Eleftheria Zeggini,I Sadaf Farooqi,Inês BarrosoPubMed 32492392
- PloS one 8:e831902013RVX-208, an inducer of ApoA-I in humans, is a BET bromodomain antagonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin G McLure,Emily M Gesner,Laura Tsujikawa,Olesya A Kharenko,Sarah Attwell,Eric Campeau,Sylwia Wasiak,Adam Stein,Andre White,Eric Fontano,Robert K Suto,Norman C W Wong,Gregory S Wagner,Henrik C Hansen,Peter R YoungPubMed 24391744
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PHIP aa 1600-1650. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WWQ0
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Bat | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Cow | Dilution info - | Notes - |
Target data
Function
Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti-apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization.
Alternative names
DCAF14, WDR11, PHIP, PH-interacting protein, DDB1- and CUL4-associated factor 14, IRS-1 PH domain-binding protein, WD repeat-containing protein 11
Recommended products
Rabbit Polyclonal PHIP antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PHIP aa 1600-1650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PHIP aa 1600-1650. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WWQ0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHIP also known as pleckstrin homology domain interacting protein is an adapter protein that interacts with insulin receptor substrates. It has a mass of approximately 100 kDa. PHIP is expressed in various tissues including the brain and liver. The protein is involved in intracellular signaling pathways by binding phosphoinositides which are important for cellular communication and growth regulation.
- Biological function summary
PHIP plays a significant role in cell proliferation and survival. It contributes to the regulation of the cell cycle by influencing transcription factors and other core components involved in cell growth. PHIP is not only a standalone protein but also part of complexes that modulate chromatin affecting gene expression patterns.
- Pathways
PHIP is integrated into insulin signaling and PI3K/AKT pathways. In these pathways PHIP interacts with key proteins like AKT1 supporting cellular responses to insulin and growth factors. The involvement of PHIP in these pathways highlights its role in metabolic regulation and its impact on cell growth and metabolism.
- Associated diseases and disorders
PHIP has links to cancer and diabetes. Its dysregulation contributes to abnormal cell proliferation often observed in cancers. PHIP's interaction with insulin signaling components such as IRS1 demonstrates its relevance to diabetes management. These connections show PHIP's potential as a therapeutic target for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-PHIP antibody (ab86244)
All lanes: Western blot - Anti-PHIP antibody (ab86244) at 0.04 µg/mL
Lane 1: Whole cell lysate from HeLa cells at 50 µg
Lane 2: Whole cell lysate from HeLa cells at 15 µg
Lane 3: Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 230 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com