Knockout Tested Rabbit Recombinant Monoclonal PHKA1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--western-blot-img104943.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913)")
![Flow Cytometry (Intracellular) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--flow-cytometry-intracellular-img79265.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54450.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913)")
![Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--western-blot-img417633.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (AB249913)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Alternative names
PHKA, PHKA1, Phosphorylase kinase alpha M subunit
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal PHKA1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12118
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249913 is the carrier-free version of Anti-PHKA1 antibody [EPR12118] ab176338.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHKA1 also known as phosphorylase kinase alpha 1 is a regulatory subunit of the phosphorylase kinase enzyme complex with a molecular mass around 138 kDa. This protein is mainly expressed in skeletal muscle and heart tissue. PHKA1 plays an important role in glycogen metabolism by regulating the conversion of phosphorylase b to phosphorylase a which ultimately leads to glycogen breakdown.
- Biological function summary
Glycogen metabolism involves PHKA1 as part of the phosphorylase kinase complex. This complex exists in the muscle and liver and includes alpha beta gamma and delta subunits. PHKA1 binds together with these subunits to form a large complex helping to regulate the activity state of the enzyme. The regulation of glycogen phosphorylase is important for maintaining energy supply in cells particularly during physical activity.
- Pathways
The activity of PHKA1 impacts glycogenolysis which is a significant metabolic pathway responsible for breaking down glycogen into glucose-1-phosphate. This pathway involves other proteins like glycogen phosphorylase and phosphoprotein phosphatase 1. PHKA1 has interactions within the cascade that ensures proper glycogen breakdown especially during conditions requiring increased energy output.
- Associated diseases and disorders
Mutations in PHKA1 are linked to glycogen storage disease type IXd (GSD IXd) which primarily affects muscle tissue. This condition features exercise intolerance and muscle weakness due to impaired glycogen breakdown. PHKB another homologous phosphorylase kinase subunit can connect to similar energy metabolism disruptions showing a clear association with the disorder pathway alongside PHKA1.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--western-blot-img104943.jpg "Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)")
Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)
This data was developed using Anti-PHKA1 antibody [EPR12118] ab176338, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PHKA1 antibody [EPR12118] (Anti-PHKA1 antibody [EPR12118] ab176338) at 1/1000 dilution
Lane 1: A431 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Human skeletal muscle lysate at 10 µg
Predicted band size: 137 kDa
![Flow Cytometry (Intracellular) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--flow-cytometry-intracellular-img79265.jpg "Flow Cytometry (Intracellular) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)")
Flow Cytometry (Intracellular) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)
This data was developed using Anti-PHKA1 antibody [EPR12118] ab176338, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized Hela cells labeling PHKA1 with Anti-PHKA1 antibody [EPR12118] ab176338 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54450.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)
This data was developed using Anti-PHKA1 antibody [EPR12118] ab176338, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKA1 with Anti-PHKA1 antibody [EPR12118] ab176338 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913), expandable thumbnail](https://content.abcam.com/products/images/phka1-antibody-epr12118-bsa-and-azide-free-ab249913--western-blot-img417633.jpg "Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)")
Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913)
This data was developed using Anti-PHKA1 antibody [EPR12118] ab176338, the same antibody clone in a different buffer formulation.
False colour image of Western blot: Anti-PHKA1 antibody [EPR12118] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-PHKA1 antibody [EPR12118] ab176338 was shown to bind specifically to PHKA1. A band was observed at 130 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in PHKA1 knockout cell line Human PHKA1 knockout HEK-293T cell line ab267337 (knockout cell lysate Human PHKA1 knockout HEK-293T cell lysate ab258111).
To generate this image, wild-type and PHKA1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.Lanes 1 - 2: Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (ab249913) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-PHKA1 antibody [EPR12118] (Anti-PHKA1 antibody [EPR12118] ab176338) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: PHKA1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human PHKA1 knockout HEK-293T cell line (Human PHKA1 knockout HEK-293T cell line ab267337)
Lane 2: Western blot - Human PHKA1 knockout HEK-293T cell lysate (Human PHKA1 knockout HEK-293T cell lysate ab258111)
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 130 kDa
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