Rabbit Recombinant Monoclonal PHKG1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179)")
![Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--western-blot-img148772.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179)")
![Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--western-blot-img62210.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36093.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103109.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (AB251179)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).
Alternative names
PHKG, PHKG1, PHK-gamma-M, Phosphorylase kinase subunit gamma-1, Serine/threonine-protein kinase PHKG1
Recommended products
Rabbit Recombinant Monoclonal PHKG1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14812
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251179 is the carrier-free version of Anti-PHKG1 antibody [EPR14812] ab194112.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHKG1 is also known as phosphorylase kinase gamma 1. PHKG1 is an enzyme that plays a role in phosphorylase kinase activity. This enzyme has a molecular mass of approximately 45 kDa. Researchers found PHKG1 expressed mainly in skeletal muscle tissue where it participates in glycogen metabolism. The enzyme contains catalytic subunits that function in phosphorylating serine and threonine residues essential for glycogen breakdown.
- Biological function summary
PHKG1 acts as a critical component of the larger phosphorylase kinase complex. This complex promotes glycogenolysis the process of converting glycogen to glucose-1-phosphate facilitating energy release in cells. Within tissues PHKG1 interacts with other subunits of phosphorylase kinase playing a regulatory role. Effective activation of phosphorylase b to active phosphorylase a form relies on efficient PHKG1 function.
- Pathways
PHKG1 is essential in the glycogenolysis pathway. It interacts with AMP-activated protein kinase (AMPK). Besides PHKG1 associates with cAMP-dependent protein kinase (PKA) as both enzymes participate in regulating energy homeostasis. These interactions highlight the enzyme’s function in maintaining metabolic balance under stress conditions.
- Associated diseases and disorders
PHKG1 associates with McArdle's disease a glycogen storage disorder characterized by muscle weakness and fatigue. Aberrant PHKG1 activity can disrupt glycogen breakdown aggravating symptoms of the condition. Moreover metabolic syndrome a cluster of metabolic disorders connects to PHKG1 through its role in regulating glucose metabolism and shares common pathways with AMPK relating PHKG1 to potential targets for therapy in metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with Anti-PHKG1 antibody [EPR14812] ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--western-blot-img148772.jpg "Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PHKG1 antibody [EPR14812] (Anti-PHKG1 antibody [EPR14812] ab194112) at 1/20000 dilution
All lanes: Human skeletal muscle tissue lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa
![Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--western-blot-img62210.jpg "Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Western blot - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PHKG1 antibody [EPR14812] (Anti-PHKG1 antibody [EPR14812] ab194112) at 1/1000 dilution
Lane 1: Mouse muscle tissue lysate at 10 µg
Lane 2: Rat muscle tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36093.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with Anti-PHKG1 antibody [EPR14812] ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103109.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with Anti-PHKG1 antibody [EPR14812] ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunocytochemistry-immunofluorescence-img67347.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)
This data was developed using Anti-PHKG1 antibody [EPR14812] ab194112, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with Anti-PHKG1 antibody [EPR14812] ab194112 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179), expandable thumbnail](https://content.abcam.com/products/images/phkg1-antibody-epr14812-bsa-and-azide-free-ab251179--immunocytochemistry-immunofluorescence-img67347.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] - BSA and Azide free (ab251179)")
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