JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133654

Anti-PHLDA1 antibody [EPR6674]

Be the first to review this product! Submit a review

|

(9 Publications)

Rabbit Recombinant Monoclonal PHLDA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

PHRIP, TDAG51, PHLDA1, Pleckstrin homology-like domain family A member 1, Apoptosis-associated nuclear protein, Proline- and glutamine-rich protein, Proline- and histidine-rich protein, T-cell death-associated gene 51 protein, PQ-rich protein, PQR protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (AB133654)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (AB133654)

Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (AB133654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (AB133654)

Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-PHLDA1 antibody [EPR6674] (AB133654)
  • WB

Unknown

Western blot - Anti-PHLDA1 antibody [EPR6674] (AB133654)

All lanes:

Western blot - Anti-PHLDA1 antibody [EPR6674] (ab133654) at 1/10000 dilution

Lane 1:

Human melanoma tissue lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 45 kDa

false

OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (AB133654)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (AB133654)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6674

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB244526

Anti-PHLDA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody (AB244526)

  • 3
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHLDA1 protein also known as T-cell death-associated gene 51 (TDAG51) weighs approximately 34 kDa. This protein plays roles in regulating apoptosis and cell proliferation. It expresses mainly in the heart kidney pancreas and placenta. PHLDA1 consists of a pleckstrin homology domain which mediates its binding to phosphoinositides allowing it to interact with cell membranes and influence cellular signaling pathways.
Biological function summary

PHLDA1 contributes to cellular processes such as apoptosis survival and proliferation. It does not appear to be part of a larger complex but engages in interactions that regulate cell fate. PHLDA1 mediates cellular response to stress and participates in cellular growth regulation by modulating the activity of other signaling molecules. This function makes it important in maintaining cellular homeostasis.

Pathways

Researchers connect PHLDA1 to the Akt signaling pathway and the TNF receptor pathway. In the Akt signaling pathway PHLDA1 interacts with proteins such as Forkhead box O3 (FOXO3) to influence cell survival and apoptosis. In the TNF receptor pathway PHLDA1 participates in modulating the balance between cell survival and programmed cell death impacting inflammatory responses.

PHLDA1 relates to cancer and neurodegenerative diseases. Its expression levels alter in certain types of cancers such as breast and prostate cancer where it may have either tumor-suppressing or oncogenic roles depending on context. In Alzheimer's disease connections exist between PHLDA1 dysregulation and proteins like amyloid precursor protein (APP) suggesting a role in disease progression. Understanding PHLDA1’s role in these conditions may offer insight into new therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
See full target information PHLDA1
style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 55:102301 PubMed40132389

2025

Single-cell sequencing reveals PHLDA1-positive smooth muscle cells promote local invasion in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Guo,Xutao Wen,Shun Yu,Jun Yang

International journal of molecular sciences 24: PubMed37047202

2023

HDAC Inhibition Restores Response to HER2-Targeted Therapy in Breast Cancer via Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha S Clayton,Edward P Carter,Abbie E Fearon,James A Heward,Lucía Rodríguez Fernández,Lina Boughetane,Edmund H Wilkes,Pedro R Cutillas,Richard P Grose

Allergologia et immunopathologia 50:1-9 PubMed36335439

2022

PHLDA1 knockdown inhibits inflammation and oxidative stress by regulating JNK/ERK pathway, and plays a protective role in sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Gong,Wenmei Liang,Qinju Lu,Jing Zhang

Cellular and molecular life sciences : CMLS 79:520 PubMed36107262

2022

PHLDA1 promotes glioblastoma cell growth via sustaining the activation state of Ras.

Applications

Unspecified application

Species

Unspecified reactive species

Jiutao Wang,Ning Yao,Yamei Hu,Mingjuan Lei,Meixian Wang,Lu Yang,Satyananda Patel,Xiang Li,Kangdong Liu,Zigang Dong

Nature communications 13:4063 PubMed35831322

2022

Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kubota,Yuko Fujioka,Ashwini Patil,Yusuke Takagi,Daisuke Matsubara,Masatomi Iijima,Isao Momose,Ryosuke Naka,Kenta Nakai,Nobuo N Noda,Mutsuhiro Takekawa

Frontiers in oncology 10:1250 PubMed32983961

2020

Circ0085539 Promotes Osteosarcoma Progression by Suppressing miR-526b-5p and PHLDA1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Liu,Wei Liu,Hang Gao,Yuanding Zhang,Ming Yan,Xu Wang

Cell reports 22:2469-2481 PubMed29490281

2018

PHLDA1 Mediates Drug Resistance in Receptor Tyrosine Kinase-Driven Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie E Fearon,Edward P Carter,Natasha S Clayton,Edmund H Wilkes,Ann-Marie Baker,Ekaterina Kapitonova,Bakhouche A Bakhouche,Yasmine Tanner,Jun Wang,Emanuela Gadaleta,Claude Chelala,Kate M Moore,John F Marshall,Juliette Chupin,Peter Schmid,J Louise Jones,Michelle Lockley,Pedro R Cutillas,Richard P Grose

Clinical and translational medicine 6:5 PubMed28101782

2017

The antineoplastic drug, trastuzumab, dysregulates metabolism in iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Brian M Necela,Bianca C Axenfeld,Daniel J Serie,Jennifer M Kachergus,Edith A Perez,E Aubrey Thompson,Nadine Norton

The Journal of biological chemistry 291:10058-66 PubMed26961871

2016

Englerin A Inhibits EWS-FLI1 DNA Binding in Ewing Sarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vittorio Caropreso,Emad Darvishi,Thomas J Turbyville,Ranjala Ratnayake,Patrick J Grohar,James B McMahon,Girma M Woldemichael
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com