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Rabbit Recombinant Monoclonal PHLDA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Target data

Function

Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.

Alternative names

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Rabbit Recombinant Monoclonal PHLDA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR6674

Purification technique

Affinity purification Protein A

Dissociation constant

3.35 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

PHLDA1 contributes to cellular processes such as apoptosis survival and proliferation. It does not appear to be part of a larger complex but engages in interactions that regulate cell fate. PHLDA1 mediates cellular response to stress and participates in cellular growth regulation by modulating the activity of other signaling molecules. This function makes it important in maintaining cellular homeostasis.

Mechanical summary

The PHLDA1 protein also known as T-cell death-associated gene 51 (TDAG51) weighs approximately 34 kDa. This protein plays roles in regulating apoptosis and cell proliferation. It expresses mainly in the heart kidney pancreas and placenta. PHLDA1 consists of a pleckstrin homology domain which mediates its binding to phosphoinositides allowing it to interact with cell membranes and influence cellular signaling pathways.

Pathway

Researchers connect PHLDA1 to the Akt signaling pathway and the TNF receptor pathway. In the Akt signaling pathway PHLDA1 interacts with proteins such as Forkhead box O3 (FOXO3) to influence cell survival and apoptosis. In the TNF receptor pathway PHLDA1 participates in modulating the balance between cell survival and programmed cell death impacting inflammatory responses.

Disease

PHLDA1 relates to cancer and neurodegenerative diseases. Its expression levels alter in certain types of cancers such as breast and prostate cancer where it may have either tumor-suppressing or oncogenic roles depending on context. In Alzheimer's disease connections exist between PHLDA1 dysregulation and proteins like amyloid precursor protein (APP) suggesting a role in disease progression. Understanding PHLDA1’s role in these conditions may offer insight into new therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PHLDA1 antibody [EPR6674] (ab133654), expandable thumbnail

    Western blot - Anti-PHLDA1 antibody [EPR6674] (ab133654)

    All lanes: Western blot - Anti-PHLDA1 antibody [EPR6674] (AB133654) at 1/10000 dilution

    Lane 1: Human melanoma tissue lysate at 10 µg

    Lane 2: A375 cell lysate at 10 µg

    Lane 3: BxPC3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (ab133654), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (ab133654)

    Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (ab133654), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (ab133654)

    Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (ab133654), expandable thumbnail

    OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (ab133654)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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