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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Recombinant Monoclonal PHLDA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | IP | Flow Cyt | WB | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
Pleckstrin homology-like domain family A member 1, Apoptosis-associated nuclear protein, Proline- and glutamine-rich protein, Proline- and histidine-rich protein, T-cell death-associated gene 51 protein, PQ-rich protein, PQR protein, TDAG51, PHRIP, PHLDA1
Rabbit Recombinant Monoclonal PHLDA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
Pleckstrin homology-like domain family A member 1, Apoptosis-associated nuclear protein, Proline- and glutamine-rich protein, Proline- and histidine-rich protein, T-cell death-associated gene 51 protein, PQ-rich protein, PQR protein, TDAG51, PHRIP, PHLDA1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
EPR6674
Affinity purification Protein A
3.35 x 10-10 M
Blue Ice
-20°C
Stable for 12 months at -20°C
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
PHLDA1 contributes to cellular processes such as apoptosis survival and proliferation. It does not appear to be part of a larger complex but engages in interactions that regulate cell fate. PHLDA1 mediates cellular response to stress and participates in cellular growth regulation by modulating the activity of other signaling molecules. This function makes it important in maintaining cellular homeostasis.
The PHLDA1 protein also known as T-cell death-associated gene 51 (TDAG51) weighs approximately 34 kDa. This protein plays roles in regulating apoptosis and cell proliferation. It expresses mainly in the heart kidney pancreas and placenta. PHLDA1 consists of a pleckstrin homology domain which mediates its binding to phosphoinositides allowing it to interact with cell membranes and influence cellular signaling pathways.
Researchers connect PHLDA1 to the Akt signaling pathway and the TNF receptor pathway. In the Akt signaling pathway PHLDA1 interacts with proteins such as Forkhead box O3 (FOXO3) to influence cell survival and apoptosis. In the TNF receptor pathway PHLDA1 participates in modulating the balance between cell survival and programmed cell death impacting inflammatory responses.
PHLDA1 relates to cancer and neurodegenerative diseases. Its expression levels alter in certain types of cancers such as breast and prostate cancer where it may have either tumor-suppressing or oncogenic roles depending on context. In Alzheimer's disease connections exist between PHLDA1 dysregulation and proteins like amyloid precursor protein (APP) suggesting a role in disease progression. Understanding PHLDA1’s role in these conditions may offer insight into new therapeutic strategies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-PHLDA1 antibody [EPR6674] (AB133654) at 1/10000 dilution
Lane 1: Human melanoma tissue lysate at 10 µg
Lane 2: A375 cell lysate at 10 µg
Lane 3: BxPC3 cell lysate at 10 µg
All lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.
Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com