AB240076

Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PHLDA1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PHRIP, TDAG51, PHLDA1, Pleckstrin homology-like domain family A member 1, Apoptosis-associated nuclear protein, Proline- and glutamine-rich protein, Proline- and histidine-rich protein, T-cell death-associated gene 51 protein, PQ-rich protein, PQR protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133654).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133654).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6674

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab240076 is the carrier-free version of ab133654.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHLDA1 protein also known as T-cell death-associated gene 51 (TDAG51) weighs approximately 34 kDa. This protein plays roles in regulating apoptosis and cell proliferation. It expresses mainly in the heart kidney pancreas and placenta. PHLDA1 consists of a pleckstrin homology domain which mediates its binding to phosphoinositides allowing it to interact with cell membranes and influence cellular signaling pathways.

Biological function summary

PHLDA1 contributes to cellular processes such as apoptosis survival and proliferation. It does not appear to be part of a larger complex but engages in interactions that regulate cell fate. PHLDA1 mediates cellular response to stress and participates in cellular growth regulation by modulating the activity of other signaling molecules. This function makes it important in maintaining cellular homeostasis.

Pathways

Researchers connect PHLDA1 to the Akt signaling pathway and the TNF receptor pathway. In the Akt signaling pathway PHLDA1 interacts with proteins such as Forkhead box O3 (FOXO3) to influence cell survival and apoptosis. In the TNF receptor pathway PHLDA1 participates in modulating the balance between cell survival and programmed cell death impacting inflammatory responses.

PHLDA1 relates to cancer and neurodegenerative diseases. Its expression levels alter in certain types of cancers such as breast and prostate cancer where it may have either tumor-suppressing or oncogenic roles depending on context. In Alzheimer's disease connections exist between PHLDA1 dysregulation and proteins like amyloid precursor protein (APP) suggesting a role in disease progression. Understanding PHLDA1’s role in these conditions may offer insight into new therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.

See full target information PHLDA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] - BSA and Azide free (AB240076)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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