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AB81464

Anti-PHLDA3 antibody [4B6]

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(3 Publications)

Mouse Monoclonal PHLDA3 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PHLDA3 aa 1-50.

View Alternative Names

TIH1, PHLDA3, Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homolog 1

1 Images
Western blot - Anti-PHLDA3 antibody [4B6] (AB81464)
  • WB

Unknown

Western blot - Anti-PHLDA3 antibody [4B6] (AB81464)

Western blotting was performed using ab81464 to detect PHLDA3 protein expression. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).

All lanes:

Western blot - Anti-PHLDA3 antibody [4B6] (ab81464)

Predicted band size: 14 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B6

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PHLDA3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5J5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Purification notes
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHLDA3 also known as PHLDA family member 3 is a small protein with a molecular mass of about 15 kDa. It functions mechanically as an antagonist of the AKT signaling pathway. PHLDA3 can inhibit AKT by binding to its pleckstrin homology (PH) domain preventing AKT's interaction with other molecules. This protein is expressed in various tissues throughout the body including the brain lungs and liver indicating its role in diverse cellular functions.
Biological function summary

The protein acts as a tumor suppressor through its ability to regulate cell growth and apoptosis. PHLDA3 does this by modulating the AKT pathway inhibiting cell survival and promoting apoptosis. It is not part of a larger complex but interacts directly with other proteins to execute its function. The influence of PHLDA3 on cell cycle processes suggests it plays roles in maintaining normal cellular functions and preventing uncontrolled proliferation.

Pathways

Several studies have shown PHLDA3 integrates into the p53 signaling pathway a critical pathway for cellular stress responses and tumor suppression. It interacts with the AKT pathway which connects it to proteins like PI3K and PTEN influencing cellular growth and apoptosis. PHLDA3 effectively bridges interaction between the p53 pathway and AKT pathway which balances cell fate decisions under stress conditions.

PHLDA3 exhibits a significant impact on cancer development and progression. Loss of PHLDA3 expression has been observed in various tumors suggesting its role in cancer development. Additionally it relates to diabetes where altered AKT signaling affects insulin response. In cancer PHLDA3 connects to the tumor suppressor protein p53 influencing its regulatory functions while in diabetes its relation to AKT pathway points to disturbances in glucose homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.
See full target information PHLDA3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:3993-4009 PubMed35112982

2022

Pleckstrin homology-like domain family A, member 3, a miR-19a-3p-regulated gene, suppresses tumor growth in osteosarcoma by downregulating the Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Yu Huang,Xin Xia,Jian Han,Lu Zhang,Wenzhi Zhao

Nucleus (Austin, Tex.) 3:200-10 PubMed22555604

2012

Phosphatidylserine colocalizes with epichromatin in interphase nuclei and mitotic chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Prudovsky,Calvin P H Vary,Yolanda Markaki,Ada L Olins,Donald E Olins

Cell 136:535-50 PubMed19203586

2009

PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Kawase,Rieko Ohki,Tatsuhiro Shibata,Shuichi Tsutsumi,Naoko Kamimura,Johji Inazawa,Tsutomu Ohta,Hitoshi Ichikawa,Hiroyuki Aburatani,Fumio Tashiro,Yoichi Taya
View all publications
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Product promise

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