JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202350

Anti-PHLDB2 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal PHLDB2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHLDB2 aa 800 to C-terminus.

View Alternative Names

LL5B, PHLDB2, Pleckstrin homology-like domain family B member 2, Protein LL5-beta

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB202350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB202350)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling PHLDB2 with ab202350 at 4 μg/ml (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB202350)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB202350)

Immunohistochemical analysis of paraffin-embedded Human stomach upper tissue labeling PHLDB2 with ab202350 at 1/20 dilution.

Western blot - Anti-PHLDB2 antibody (AB202350)
  • WB

Supplier Data

Western blot - Anti-PHLDB2 antibody (AB202350)

Recommended conditions :

Dilution : 1 : 100 - 1 : 250

All lanes:

Western blot - Anti-PHLDB2 antibody (ab202350)

All lanes:

Human cell line U-2 OS

Predicted band size: 142 kDa

Observed band size: 142 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PHLDB2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86SQ0

style
left-column

Alternative Products

Primary Antibodies

AB234885

Anti-PHLDB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB234885)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

  • 5
  • 38
Primary Antibodies

AB16056

Anti-COX IV antibody - Mitochondrial Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

  • 5
  • 33
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB235598

Anti-SPCS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPCS3 antibody (AB235598)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PHLDB2 also known as pleckstrin homology-like domain family B member 2 plays important mechanical roles in cellular signaling and structural processes. It weighs around 122 kDa and expresses in various tissues including the brain and immune cells. PHLDB2 interacts with the plasma membrane through its pleckstrin homology domain playing a regulatory role in cytoskeletal arrangements and cellular adhesion.
Biological function summary

The protein interacts within cellular environments to modulate cell polarity and migration often involving complex formations. PHLDB2 associates with actin filaments and contributes to directed cell movement. It helps regulate immune responses by influencing antibody production and cell surface receptor dynamics impacting how cells respond to external signals.

Pathways

PHLDB2 participates actively in the PI3K/AKT signaling pathway and cytoskeleton regulation. It partners with other proteins like AKT to control cell growth survival and metabolism. The protein also interacts within the Wnt signaling pathway aiding in further modulation of cellular polarization and movement linking it to cell fate decisions in development.

PHLDB2 is linked to glioblastoma and systemic lupus erythematosus through its influence on cellular migration and immune regulation. In glioblastoma aberrant expression levels of PHLDB2 affect tumor cell invasion and metastasis often involving interactions with proteins such as integrins. In systemic lupus erythematosus PHLDB2 dysregulation impacts immune cell functions and inflammatory responses connecting to proteins like IL-6 in the immune signaling pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane (By similarity).
See full target information PHLDB2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed36828547

2023

Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Hurwitz,Parash Parajuli,Seval Ozkan,Celine Prunier,Thien Ly Nguyen,Deanna Campbell,Creighton Friend,Allyn Austin Bryan,Ting-Xuan Lu,Steven Christopher Smith,Mohammed Shawkat Razzaque,Keli Xu,Azeddine Atfi

Oncogene : PubMed33452458

2021

NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Kang,Jinglin Zhang,Tingting Huang,Yuhang Zhou,Chi Chun Wong,Ronald C K Chan,Yujuan Dong,Feng Wu,Bin Zhang,William K K Wu,Michael W Y Chan,Alfred S L Cheng,Jun Yu,Nathalie Wong,Kwok Wai Lo,Ka Fai To
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com