AB234885

Anti-PHLDB2 antibody

Name: Anti-PHLDB2 antibody (ab234885) | Abcam
Brand: Abcam Limited
SKU: AB234885
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PHLDB2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHLDB2 aa 700-1050.

View Alternative Names

LL5B, PHLDB2, Pleckstrin homology-like domain family B member 2, Protein LL5-beta

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB234885)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB234885)

Paraffin-embedded human gastric cancer tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB234885)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB234885)

HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PHLDB2 using ab234885 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB234885)

Paraffin-embedded human adrenal gland tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PHLDB2 antibody (AB234885)

All lanes:

Western blot - Anti-PHLDB2 antibody (ab234885) at 1/500 dilution

All lanes:

Mouse liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 142 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PHLDB2 aa 700-1050. The exact immunogen used to generate this antibody is proprietary information.

Q86SQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PHLDB2 also known as pleckstrin homology-like domain family B member 2 plays important mechanical roles in cellular signaling and structural processes. It weighs around 122 kDa and expresses in various tissues including the brain and immune cells. PHLDB2 interacts with the plasma membrane through its pleckstrin homology domain playing a regulatory role in cytoskeletal arrangements and cellular adhesion.

Biological function summary

The protein interacts within cellular environments to modulate cell polarity and migration often involving complex formations. PHLDB2 associates with actin filaments and contributes to directed cell movement. It helps regulate immune responses by influencing antibody production and cell surface receptor dynamics impacting how cells respond to external signals.

Pathways

PHLDB2 participates actively in the PI3K/AKT signaling pathway and cytoskeleton regulation. It partners with other proteins like AKT to control cell growth survival and metabolism. The protein also interacts within the Wnt signaling pathway aiding in further modulation of cellular polarization and movement linking it to cell fate decisions in development.

PHLDB2 is linked to glioblastoma and systemic lupus erythematosus through its influence on cellular migration and immune regulation. In glioblastoma aberrant expression levels of PHLDB2 affect tumor cell invasion and metastasis often involving interactions with proteins such as integrins. In systemic lupus erythematosus PHLDB2 dysregulation impacts immune cell functions and inflammatory responses connecting to proteins like IL-6 in the immune signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane (By similarity).

See full target information PHLDB2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 11:1470-1481 PubMed36247888

2022

Inflammatory factor tumor necrosis factor-α (TNF-α) activates P-glycoprotein (P-gp) by phosphorylating c-Jun and thus promotes transportation in placental cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huaying Li,Li Yan,Biao Li,Guoqing Wei,Rong Ju

Frontiers in cell and developmental biology 9:688949 PubMed34746118

2021

NR2F1-AS1/miR-190a/PHLDB2 Induces the Epithelial-Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3.

Applications

Unspecified application

Species

Unspecified reactive species

Jinqi Lv,Simeng Zhang,Yang Liu,Ce Li,Tianshu Guo,Shuairan Zhang,Zenan Li,Zihan Jiao,Haina Sun,Ye Zhang,Ling Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHLDB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB234885)

Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (AB234885)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (AB234885)

Western blot - Anti-PHLDB2 antibody (AB234885)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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