Rabbit Recombinant Monoclonal SCOP antibody. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | IP | |
---|---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
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Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (PubMed:15808505, PubMed:17386267, PubMed:18162466). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation (PubMed:18162466). Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor (PubMed:19079341). Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (By similarity). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (By similarity). Involved in development and function of regulatory T-cells (By similarity).
KIAA0606, PHLPP, PLEKHE1, SCOP, PHLPP, PLEKHE1, SCOP, PHLPP1, KIAA0606, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein, PH domain-containing family E member 1, hSCOP
Rabbit Recombinant Monoclonal SCOP antibody. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27151-55
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
SCOP also known as Secretory Carrier Membrane Protein plays an important role in the endocytic and exocytic transport processes. This protein weighing about 28 kDa is expressed in a variety of tissues including the brain lung and pancreas. It acts within cellular vesicles to mediate the secretion and uptake of essential molecules. These activities enable the cell to maintain material exchange which is important for normal cellular functions and communication.
This protein takes part in intracellular transport helping in material distribution throughout the cell. SCOP associates with vesicular transport machinery components assisting in the precise navigation and fusion of vesicles. Through this association SCOP helps in the release of neurotransmitters and hormones affecting key cellular signaling processes. Its involvement in exocytosis renders it a part of the complex system overseeing cellular secretion.
SCOP becomes integral in the regulation of vesicle trafficking. Its contributions are evident within two pathways: synaptic vesicle cycle and insulin secretion pathway. In conjunction with SNARE proteins SCOP aids in both docking and fusion during vesicle transport. These pathways permit smooth execution of neurotransmitter release and insulin secretion which are vital for neurological and metabolic processes respectively.
Dysregulation of SCOP associates with neurological disorders and metabolic diseases. Improper function of SCOP can impair neurotransmitter release leading to conditions like epilepsy. On the other hand aberrations in insulin secretion due to SCOP malfunction can contribute to diabetes mellitus. SCOP's interaction with other proteins like synaptotagmin during vesicle fusion highlights its importance across these diseases by impacting the vesicle lifecycle and associated metabolic outcomes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 25827478, PMID: 20819118).
Exposure time: 81 seconds
All lanes: Western blot - Anti-PHLPP1 antibody [EPR27151-55] (ab305295) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 3: HEK-293 transfected with siRNA specifically targeti PHLPP1 whole cell lysate 20 at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa, 184 kDa
Exposure time: 81s
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