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AB305295

Anti-PHLPP1 antibody [EPR27151-55]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SCOP antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0606, PHLPP, PLEKHE1, SCOP, PHLPP1, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein, PH domain-containing family E member 1, hSCOP

2 Images
Western blot - Anti-PHLPP1 antibody [EPR27151-55] (AB305295)
  • WB

Lab

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (AB305295)

Western blot : Anti-PHLPP1 antibody [EPR27151-55] ab305295 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 160-200 kDa in Wild-type A549 cell lysates with no signal observed at this size in PHLPP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (ab305295) at 1/1000 dilution

Lane 1:

Wild-type A549 at 40 µg

Lane 2:

Western blot - Human PHLPP1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-phlpp1-knockout-a549-cell-line-ab287700'>ab287700</a>) at 40 µg

Lane 3:

HEK-293 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 185 kDa

Observed band size: 160-200 kDa,77 kDa

false

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (AB305295)
  • WB

Supplier Data

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (AB305295)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID : 25827478, PMID : 20819118). Exposure time : 81 seconds

All lanes:

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (ab305295) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 3:

HEK-293 transfected with siRNA specifically targeti PHLPP1 whole cell lysate 20 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa,184 kDa

false

Exposure time: 81s

  • Carrier free

    Anti-PHLPP1 antibody [EPR27151-55] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27151-55

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCOP also known as Secretory Carrier Membrane Protein plays an important role in the endocytic and exocytic transport processes. This protein weighing about 28 kDa is expressed in a variety of tissues including the brain lung and pancreas. It acts within cellular vesicles to mediate the secretion and uptake of essential molecules. These activities enable the cell to maintain material exchange which is important for normal cellular functions and communication.
Biological function summary

This protein takes part in intracellular transport helping in material distribution throughout the cell. SCOP associates with vesicular transport machinery components assisting in the precise navigation and fusion of vesicles. Through this association SCOP helps in the release of neurotransmitters and hormones affecting key cellular signaling processes. Its involvement in exocytosis renders it a part of the complex system overseeing cellular secretion.

Pathways

SCOP becomes integral in the regulation of vesicle trafficking. Its contributions are evident within two pathways: synaptic vesicle cycle and insulin secretion pathway. In conjunction with SNARE proteins SCOP aids in both docking and fusion during vesicle transport. These pathways permit smooth execution of neurotransmitter release and insulin secretion which are vital for neurological and metabolic processes respectively.

Dysregulation of SCOP associates with neurological disorders and metabolic diseases. Improper function of SCOP can impair neurotransmitter release leading to conditions like epilepsy. On the other hand aberrations in insulin secretion due to SCOP malfunction can contribute to diabetes mellitus. SCOP's interaction with other proteins like synaptotagmin during vesicle fusion highlights its importance across these diseases by impacting the vesicle lifecycle and associated metabolic outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (PubMed : 15808505, PubMed : 17386267, PubMed : 18162466). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation (PubMed : 18162466). Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor (PubMed : 19079341). Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606). May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (By similarity). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (By similarity). Involved in development and function of regulatory T-cells (By similarity).
See full target information PHLPP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Osteoarthritis and cartilage open 7:100525 PubMed39811690

2025

Postnatal deletion of Phlpp1 in chondrocytes delays post-traumatic osteoarthritis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha R Weaver,Katherine M Arnold,Eduardo Peralta-Herrera,Manuela Oviedo,Elizabeth L Zars,Elizabeth W Bradley,Jennifer J Westendorf
View all publications
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