AB71973

Anti-PHLPP2 antibody

Name: Anti-PHLPP2 antibody (ab71973) | Abcam
Brand: Abcam Limited
SKU: AB71973
Price: 525 USD
Availability: InStock

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(20 Publications)

Rabbit Polyclonal PHLPP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human PHLPP2.

View Alternative Names

KIAA0931, PHLPPL, PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

2 Images

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Immunoprecipitation - Anti-PHLPP2 antibody (AB71973)

Detection of PHLPP2 by Western Blot of Immunoprecipitate.
ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PHLPP2 antibody (ab71973)

Predicted band size: 147 kDa

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Western blot - Anti-PHLPP2 antibody (AB71973)

All lanes:

Western blot - Anti-PHLPP2 antibody (ab71973) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 147 kDa

Observed band size: 190 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PHLPP2. The exact immunogen used to generate this antibody is proprietary information.

Q6ZVD8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": " for 1-4 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHLPP2 also known as PH domain leucine-rich repeat protein phosphatase 2 functions as a serine/threonine phosphatase that specifically dephosphorylates Akt at the Ser473 site. This regulatory action on Akt influences many cellular processes including cell growth survival and metabolism. PHLPP2 has a molecular mass of approximately 140 kDa and is expressed in various tissues including liver and lung. Alternative names such as SCOP2 may sometimes reference this protein in specific research contexts.

Biological function summary

PHLPP2 modulates key signaling pathways by acting as a significant negative regulator of the Akt signaling pathway. It does not work alone; PHLPP2 can interact with functional complexes to exert its phosphatase activity more effectively. By dephosphorylating Akt PHLPP2 reduces the signaling output that leads to cell proliferation and survival responses leading to normal cellular homeostasis. This regulatory function positions PHLPP2 as a balancing component ensuring that Akt activity remains tightly controlled.

Pathways

PHLPP2 plays a role in the PI3K/Akt signaling pathway which is critical for cellular growth and metabolic functions. It also indirectly impacts the mTOR pathway through its regulation of Akt as Akt is an upstream activator of mTOR. By keeping Akt activity in check via dephosphorylation PHLPP2 keeps the downstream effects of the PI3K/Akt/mTOR axis in balance. It relates closely to proteins like PTEN which also acts as a negative regulator of the PI3K/Akt pathway ensuring that excessive Akt activity does not drive uncontrolled cellular events.

Mutations or altered expression of PHLPP2 have connections to cancer and diabetes. In cancer decreased levels of PHLPP2 can lead to an increase in Akt activity promoting uncontrolled cell division and survival therefore contributing to tumorigenesis. PHLPP2's influence on diabetes involves its regulation of insulin signaling where dysregulation can affect glucose homeostasis. The relationship between PHLPP2 Akt and insulin signaling highlights its important role in maintaining normal metabolic control and preventing disease progression in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606).

See full target information PHLPP2

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14116 PubMed35982137

2022

Novel mechanisms underlying inhibition of inflammation-induced angiogenesis by dexamethasone and gentamicin via PI3K/AKT/NF-κB/VEGF pathways in acute radiation proctitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yousong Li,Qin Ding,Jinsheng Gao,Chunxia Li,Pengxiao Hou,Jie Xu,Kaiqi Cao,Min Hu,Lin Cheng,Xixing Wang,Xiaoling Yang

Cell death & disease 13:516 PubMed35654777

2022

Transcription factor SNAI2 exerts pro-tumorigenic effects on glioma stem cells via PHLPP2-mediated Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lilei Peng,Jie Fu,Yitian Chen,Yang Ming,Haiping He,Shan Zeng,Chuanhong Zhong,Ligang Chen

Nature communications 13:3056 PubMed35650206

2022

Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

R C Nayak,K H Chang,A K Singh,M Kotliar,M Desai,A M Wellendorf,M Wunderlich,J Bartram,B Mizukawa,M Cuadrado,P Dexheimer,A Barski,X R Bustelo,N N Nassar,J A Cancelas

International journal of biological sciences 18:2833-2850 PubMed35541896

2022

ZDHHC22-mediated mTOR palmitoylation restrains breast cancer growth and endocrine therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Jiefeng Huang,Jie Li,Jun Tang,Yushen Wu,Fengsheng Dai,Ziying Yi,Yan Wang,Yunhai Li,Yue Wu,Guosheng Ren,Tingxiu Xiang

Cell death & disease 13:285 PubMed35354796

2022

TRIM46 activates AKT/HK2 signaling by modifying PHLPP2 ubiquitylation to promote glycolysis and chemoresistance of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jicheng Tantai,Xufeng Pan,Yong Chen,Yuzhou Shen,Chunyu Ji

Gastroenterology 162:2004-2017.e2 PubMed35176309

2022

Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuhui Shi,Jingxuan Yang,Mingyang Liu,Yuqing Zhang,Zhijun Zhou,Wenyi Luo,Kar-Ming Fung,Chao Xu,Michael S Bronze,Courtney W Houchen,Min Li

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34921114

2021

N6-methyladenosine (mA) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Xuan Jin,Rujuan Zuo,Konstantinos Anastassiadis,Arne Klungland,Carsten Marr,Adam Filipczyk

Biotechnology and applied biochemistry 69:735-748 PubMed33734482

2021

CircRNA circ_0006892 regulates miR-24/PHLPP2 axis to mitigate cigarette smoke extract-induced bronchial epithelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chenying Zhang,Shuangshuang Gu,Xiuwen Kang

Cancer management and research 12:11341-11350 PubMed33204152

2020

Promotes Colon Cancer Cell Proliferation by Targeted Expression Inhibitionn.

Applications

Unspecified application

Species

Unspecified reactive species

Fazhuang Fang,Ling Cheng,Xiaotang Wu,Minfeng Ye,Huizhong Zhang

Acta pharmacologica Sinica : PubMed32694755

2020

MicroRNA-302a promotes neointimal formation following carotid artery injury in mice by targeting PHLPP2 thus increasing Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ying Liu,Xiu Liu,Jia-Guo Zhou,Si-Jia Liang

View all publications

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Product promise

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Anti-PHLPP2 antibody

Immunoprecipitation - Anti-PHLPP2 antibody (AB71973)

Western blot - Anti-PHLPP2 antibody (AB71973)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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