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AB153918

Anti-PHLPP2 antibody - N-terminal

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal PHLPP2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PHLPP2 aa 1-250.

View Alternative Names

KIAA0931, PHLPPL, PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PHLPP2 antibody - N-terminal (AB153918)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHLPP2 antibody - N-terminal (AB153918)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PHLPP2 with ab153918 at 1/500 dilution. Lower image is co-stained with Hoechst 33342.

Western blot - Anti-PHLPP2 antibody - N-terminal (AB153918)
  • WB

Unknown

Western blot - Anti-PHLPP2 antibody - N-terminal (AB153918)

5% SDS PAGE

All lanes:

Western blot - Anti-PHLPP2 antibody - N-terminal (ab153918) at 1/2000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 147 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PHLPP2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q6ZVD8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHLPP2 also known as PH domain leucine-rich repeat protein phosphatase 2 functions as a serine/threonine phosphatase that specifically dephosphorylates Akt at the Ser473 site. This regulatory action on Akt influences many cellular processes including cell growth survival and metabolism. PHLPP2 has a molecular mass of approximately 140 kDa and is expressed in various tissues including liver and lung. Alternative names such as SCOP2 may sometimes reference this protein in specific research contexts.
Biological function summary

PHLPP2 modulates key signaling pathways by acting as a significant negative regulator of the Akt signaling pathway. It does not work alone; PHLPP2 can interact with functional complexes to exert its phosphatase activity more effectively. By dephosphorylating Akt PHLPP2 reduces the signaling output that leads to cell proliferation and survival responses leading to normal cellular homeostasis. This regulatory function positions PHLPP2 as a balancing component ensuring that Akt activity remains tightly controlled.

Pathways

PHLPP2 plays a role in the PI3K/Akt signaling pathway which is critical for cellular growth and metabolic functions. It also indirectly impacts the mTOR pathway through its regulation of Akt as Akt is an upstream activator of mTOR. By keeping Akt activity in check via dephosphorylation PHLPP2 keeps the downstream effects of the PI3K/Akt/mTOR axis in balance. It relates closely to proteins like PTEN which also acts as a negative regulator of the PI3K/Akt pathway ensuring that excessive Akt activity does not drive uncontrolled cellular events.

Mutations or altered expression of PHLPP2 have connections to cancer and diabetes. In cancer decreased levels of PHLPP2 can lead to an increase in Akt activity promoting uncontrolled cell division and survival therefore contributing to tumorigenesis. PHLPP2's influence on diabetes involves its regulation of insulin signaling where dysregulation can affect glucose homeostasis. The relationship between PHLPP2 Akt and insulin signaling highlights its important role in maintaining normal metabolic control and preventing disease progression in these contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed : 20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed : 21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed : 24530606).
See full target information PHLPP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40801656

2025

Notch2 Deletion Compromises Epithelial Integrity and Enamel Formation in Rodent Incisors.

Applications

Unspecified application

Species

Unspecified reactive species

Argyro Lamprou,Cristina Porcheri,Thimios A Mitsiadis
View all publications
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Product promise

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