AB113688

Anti-PHO8 antibody [1D3A10]

Name: Anti-PHO8 antibody [1D3A10] (ab113688) | Abcam
Brand: Abcam Limited
SKU: AB113688

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(18 Publications)

Mouse Monoclonal PPB antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 18 publications.

View Alternative Names

YDR481C, D8035.24, PHO8, Repressible alkaline phosphatase, Membrane-bound repressible alkaline phosphatase

1 Images

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Western blot - Anti-PHO8 antibody [1D3A10] (AB113688)

All lanes:

Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)

All lanes:

Yeast whole cell lysate at 15 µg

Predicted band size: 63 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1D3A10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'PHO8' also known as yeast alkaline phosphatase is an enzyme that plays an important role in phosphate metabolism in Saccharomyces cerevisiae. PHO8 is a homodimer with a mass of approximately 70 kDa per subunit. Its expression predominantly occurs in the vacuole where it functions optimally in an acidic environment. The enzyme facilitates the dephosphorylation processes by removing phosphate groups from various substrates making it essential for phosphate salvage.

Biological function summary

PHO8 is critical for cellular phosphate homeostasis. It operates as part of a larger complex situated in the vacuole membrane. The enzyme assists in breaking down phosphomonoesters into inorganic phosphate which can then be reused by the cell. This activity is particularly important under conditions of nutrient limitation where maintaining phosphate balance becomes important. PHO8's regulation is tightly linked to environmental phosphate levels ensuring that phosphate is efficiently managed within the cell.

Pathways

PHO8 is integral to the phosphate regulatory (PHO) signaling pathway. In this network the enzyme cooperates with proteins like PHO4 and PHO2 to regulate phosphate uptake and storage. The coordination between these proteins enables the cell to adjust phosphate metabolism according to availability. Additionally PHO8 also interacts with related pathways involving the vacuole's function in storage and turnover of phosphate compounds further emphasizing its role in cellular nutrient management.

PHO8's influence extends to conditions impacting phosphate metabolism. Although largely studied in yeast insights from PHO8 functionality contribute to understanding human disorders related to phosphate dysregulation such as hypophosphatasia. Interestingly studies suggest that homologous human alkaline phosphatases share a similar regulatory mechanism influenced by the protein PHO85 in yeast shedding light on potential parallels in disease presentation and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase (PubMed : 1848184).

See full target information PHO8

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Autophagy reports 4:2511724 PubMed40458443

2025

A plasmid module for PCR-based gene modification for the accurate measurement of vacuolar delivery of specific proteins in yeast .

Applications

Unspecified application

Species

Unspecified reactive species

Jakob Valdbjørn Kanne,Fulvio Reggiori

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

The EMBO journal 44:1774-1803 PubMed39920309

2025

Reprograming of the ubiquitin ligase Ubr1 by intrinsically disordered Roq1 through cooperating multifunctional motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Niklas Peters,Sibylle Kanngießer,Oliver Pajonk,Rafael Salazar Claros,Petra Hubbe,Axel Mogk,Sebastian Schuck

Methods in enzymology 700:77-104 PubMed38971613

2024

Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Israel Juarez-Contreras,Itay Budin

The Journal of biological chemistry 300:107274 PubMed38588809

2024

Ykt6 functionally overlaps with vacuolar and exocytic R-SNAREs in the yeast Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Hayate Watanabe,Shingo Urano,Nozomi Kikuchi,Yurika Kubo,Ayumi Kikuchi,Katsuya Gomi,Takahiro Shintani

The Journal of biological chemistry 300:105496 PubMed38013088

2023

Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Itay Budin

The Journal of cell biology 222: PubMed37917025

2023

The mechanism of Atg15-mediated membrane disruption in autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Kagohashi,Michiko Sasaki,Alexander I May,Tomoko Kawamata,Yoshinori Ohsumi

STAR protocols 4:101935 PubMed36520633

2022

Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Sakamaki,Noboru Mizushima

The Journal of biological chemistry 298:102641 PubMed36306824

2022

A method for the isolation and characterization of autophagic bodies from yeast provides a key tool to investigate cargos of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Kawamata,Shiho Makino,Yoko Kagohashi,Michiko Sasaki,Yoshinori Ohsumi

PLoS genetics 18:e1010446 PubMed36215320

2022

A two-tiered system for selective receptor and transporter protein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Kathleen Golden,Thomas David Daniel Kazmirchuk,Erin Kate McNally,Mariyam El Eissawi,Zeynep Derin Gokbayrak,Joël Denis Richard,Christopher Leonard Brett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHO8 antibody [1D3A10]

Western blot - Anti-PHO8 antibody [1D3A10] (AB113688)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise