Mouse Monoclonal PPB antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications.
Images
![Western blot - Anti-PHO8 antibody [1D3A10] (AB113688), expandable thumbnail](https://content.abcam.com/products/images/pho8-antibody-1d3a10-ab113688--western-blot-img133576.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PHO8 antibody [1D3A10] (AB113688)")
Publications
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- The Journal of biological chemistry 298:1026412022A method for the isolation and characterization of autophagic bodies from yeast provides a key tool to investigate cargos of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoko Kawamata,Shiho Makino,Yoko Kagohashi,Michiko Sasaki,Yoshinori OhsumiPubMed 36306824
- PLoS genetics 18:e10104462022A two-tiered system for selective receptor and transporter protein degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Kathleen Golden,Thomas David Daniel Kazmirchuk,Erin Kate McNally,Mariyam El Eissawi,Zeynep Derin Gokbayrak,Joël Denis Richard,Christopher Leonard BrettPubMed 36215320
- Nature communications 12:23162021Selectivity of mRNA degradation by autophagy in yeast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiho Makino,Tomoko Kawamata,Shintaro Iwasaki,Yoshinori OhsumiPubMed 33875662
- PloS one 15:e02324762020Reduction of the P5A-ATPase Spf1p phosphoenzyme by a Ca2+-dependent phosphatase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerardo R Corradi et. al.PubMed 32353073
- Metallomics : integrated biometal science 11:1298-13092019Isolated Saccharomyces cerevisiae vacuoles contain low-molecular-mass transition-metal polyphosphate complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang Q Nguyen,Nathaniel Dziuba,Paul A LindahlPubMed 31210222
- Nature communications 9:53582018The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin Kate McNally et. al.PubMed 30560896
- Molecular cell 70:1025-1037.e52018SHRED Is a Regulatory Cascade that Reprograms Ubr1 Substrate Specificity for Enhanced Protein Quality Control during Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamas Szoradi et. al.PubMed 29861160
- Journal of cell science 130:3891-39062017Lipid-dependent regulation of exocytosis in by OSBP homolog (Osh) 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard J Smindak et. al.PubMed 28993464
- Aging 5:551-742013Mitochondrial membrane lipidome defines yeast longevity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Beach,Vincent R Richard,Anna Leonov,Michelle T Burstein,Simon D Bourque,Olivia Koupaki,Mylène Juneau,Rachel Feldman,Tatiana Iouk,Vladimir I TitorenkoPubMed 23924582
- Molecular and cellular biology 33:526-422012Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSurya P Manandhar,Florante Ricarte,Stephanie M Cocca,Editte GharakhanianPubMed 23166297
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Saccharomyces cerevisiae | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 4 µg/mL | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 20 µg/mL | Notes - |
Target data
Function
Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase (PubMed:1848184).
Targets
PHO8
Alternative names
Repressible alkaline phosphatase, Membrane-bound repressible alkaline phosphatase, D8035.24, YDR481C, PHO8
Recommended products
Mouse Monoclonal PPB antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications.
Alternative names
Repressible alkaline phosphatase, Membrane-bound repressible alkaline phosphatase, D8035.24, YDR481C, PHO8
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
1D3A10
- Purification technique
Proprietary technique
- Light chain type
kappa
- Concentration
- Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PHO8 antibody [1D3A10] (ab113688), expandable thumbnail](https://content.abcam.com/products/images/pho8-antibody-1d3a10-ab113688--western-blot-img133576.jpg "Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)")
Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)
All lanes: Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)
All lanes: Yeast whole cell lysate at 15 µg
Predicted band size: 63 kDa
Downloads
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