Rabbit Polyclonal Phosphatidic acid phosphatase type 2B antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLPP3 aa 250 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLPP3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14495
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P (PubMed:27694435, PubMed:9607309, PubMed:9705349). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound (PubMed:9607309). Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed:23591818, PubMed:27694435, PubMed:9607309). Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines (PubMed:27694435). By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers (PubMed:23591818). Independently of this phosphatase activity may also function in the Wnt signaling pathway and the stabilization of beta-catenin/CTNNB1, thereby regulating cell proliferation, migration and differentiation in angiogenesis or yet in tumor growth (PubMed:20123964, PubMed:21569306). Also plays a role in integrin-mediated cell-cell adhesion in angiogenesis (PubMed:12660161, PubMed:16099422).
Alternative names
LPP3, PPAP2B, PLPP3, Phospholipid phosphatase 3, Lipid phosphate phosphohydrolase 3, PAP2-beta, Phosphatidate phosphohydrolase type 2b, Phosphatidic acid phosphatase 2b, Vascular endothelial growth factor and type I collagen-inducible protein, PAP-2b, PAP2b, VCIP
Recommended products
Rabbit Polyclonal Phosphatidic acid phosphatase type 2B antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLPP3 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLPP3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14495
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphatidic acid phosphatase type 2B also known as PAP2B or Lipin-2 plays an important role in lipid metabolism by converting phosphatidic acid to diacylglycerol. This enzyme has a molecular mass of about 47 kDa. It is mainly expressed in liver brain and adipose tissues playing a significant role in regulating lipid levels within these areas. Its activity affects the production and breakdown of fats indicating its importance in metabolic processes.
- Biological function summary
This enzyme mediates lipid homeostasis and energy metabolism which are critical for cellular functions. It does not form part of a complex but works by reducing phosphatidic acid levels influencing the signaling pathways that control cell growth and differentiation. By managing lipid synthesis and degradation it helps maintain an equilibrium in lipid-related signaling processes. The conversion it catalyzes influences membrane dynamics and organelle formation.
- Pathways
The enzyme actively participates in the glycerolipid biosynthesis and insulin signaling pathways. In the glycerolipid biosynthesis pathway it interacts with related enzymes like acyl-CoA synthetase and diacylglycerol acyltransferase to regulate fatty acid processing. Its role in the insulin signaling pathway affects glucose uptake by modulating lipid signals that dictate metabolic responses to insulin aligning with other vital metabolic proteins like AMPK.
- Associated diseases and disorders
Abnormal levels or function of phosphatidic acid phosphatase type 2B have links to metabolic disorders such as obesity and type 2 diabetes. This enzyme also relates to familial partial lipodystrophy which involves abnormal fat deposition in the body. Its connection to these conditions involves proteins such as perilipin which regulates lipid storage and adiponectin which is involved in insulin sensitivity further demonstrating its significance in lipid and glucose metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Phosphatidic acid phosphatase type 2B antibody (ab221918)
PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for phosphatidic acid phosphatase type 2B (green) using ab221918 (4 μg/ml) in ICC/IF.
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