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AB225957

Anti-Phosphinotricin Acetyl Transferase antibody

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(1 Publication)

Rabbit Polyclonal PAT antibody. Suitable for WB and reacts with Recombinant full length protein - Streptomyces viridochromogenes samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Streptomyces viridochromogenes DSM 40736 pat.

View Alternative Names

Phosphinothricin N-acetyltransferase, PPT N-acetyltransferase, Phosphinothricin-resistance protein, pat

1 Images
Western blot - Anti-Phosphinotricin Acetyl Transferase antibody (AB225957)
  • WB

Supplier Data

Western blot - Anti-Phosphinotricin Acetyl Transferase antibody (AB225957)

This antibody has only been tested in WB using the recombinant protein, we have no data for endogenous protein.

All lanes:

Western blot - Anti-Phosphinotricin Acetyl Transferase antibody (ab225957) at 1/500 dilution

Lane 1:

Recombinant protein 60 ng

Lane 2:

Recombinant protein 30 ng

Lane 3:

Recombinant protein 15 ng

Lane 4:

Recombinant protein 7.5 ng

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 36 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Streptomyces viridochromogenes

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Streptomyces viridochromogenes DSM 40736 pat.

Q57146

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Streptomyces viridochromogenes": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p>This antibody has only been tested in WB using the recombinant protein, we have no data for endogenous protein.</p>" }, "Streptomyces viridochromogenes": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphinotricin Acetyl Transferase also known as PAT serves a mechanical role in the inactivation of phosphinothricin an active component in some herbicides. PAT catalyzes the transfer of an acetyl group from acetyl-CoA to phosphinothricin effectively neutralizing it. The approximate mass of PAT is 21 kDa. It is often expressed in genetically modified crops enabling them to tolerate certain herbicides that include phosphinothricin as an active ingredient.
Biological function summary

Phosphinotricin acetyl transferase contributes to herbicide resistance. It ensures the detoxification process allowing plants to grow even in the presence of herbicides that inhibit glutamine synthetase. PAT is not typically known to form part of a larger complex like some other enzymes but its activity is important for maintaining plant viability under herbicide pressure. The expression of this enzyme in plants is a form of an inducible stress response to environmental changes.

Pathways

Phosphinotricin acetyl transferase plays an important role in the metabolic pathway for xenobiotic detoxification. This enzyme is pivotal in the acetylation pathway aiding in counteracting herbicide action. PAT works alongside other metabolic enzymes facilitating detoxification although it is not directly interlinked with common metabolic proteins its function highlights the synergistic nature of plant metabolic systems when dealing with external chemical stresses.

Phosphinotricin acetyl transferase's application relates to agricultural practices rather than direct human diseases although indirectly it could affect human health due to its role in food crop production. Increased use of PAT-expressing crops might influence patterns of herbicide use with implications for biodiversity and ecosystem health. No direct connections to specific human-related protein interactions exist as PAT functions and impacts are largely associated with plant biology and crop engineering rather than mammalian systems.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.
See full target information pat

Additional targets

Phosphinotricin Acetyl Transferase

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 15:73 PubMed35718769

2022

Initiation and elongation factor co-expression correlates with recurrence and survival in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Sobočan,Daniela Brunialti,Sussanne Sprung,Christoph Schatz,Jure Knez,Rajko Kavalar,Iztok Takač,Johannes Haybaeck
View all publications

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