AB101520

Anti-Phospho1 antibody

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(1 Publication)

Rabbit Polyclonal Phospho1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PHOSPHO1 aa 1 to C-terminus.

View Alternative Names

Phosphoethanolamine/phosphocholine phosphatase, PHOSPHO1

1 Images

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Western blot - Anti-Phospho1 antibody (AB101520)

12% SDS-PAGE

All lanes:

Western blot - Anti-Phospho1 antibody (ab101520) at 1/500 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human PHOSPHO1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TCT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospho1 also known as PHOSPHO1 is a phosphatase with a molecular weight of approximately 32 kDa. It exhibits high phosphatase activity specific to phosphoethanolamine and phosphocholine. The enzyme localizes mainly in the ossification centers of developing bones particularly in osteoblasts and chondrocytes reflecting its significant role in skeletal mineralization.

Biological function summary

Phospho1 contributes to the mineralization process in bone and cartilage tissue. It plays an integral role in providing inorganic phosphate necessary for hydroxyapatite crystal formation an essential component of the mineral matrix. Phospho1 functions independently although its activity aligns with other enzymes and proteins involved in the mineralization process indicating its singular yet important enzymatic action in this biological context.

Pathways

Phospho1 is directly involved in the skeletal development and bone mineralization pathways. It interacts closely within the matrix vesicle-mediated mineralization pathway. One notable related protein is alkaline phosphatase which cooperates to supply phosphate ions for matrix mineralization. This collaboration ensures effective hydroxyapatite crystal deposition thereby maintaining bone integrity and function.

Phospho1 associates closely with conditions such as hypophosphatasia and osteoporosis. Its malfunction or dysregulation can cause impaired skeletal mineralization leading to these bone-related ailments. In the context of these conditions Phospho1 activity is linked to alkaline phosphatase as perturbation in either enzyme can result in altered bone mineral density and defective bone mineralization highlighting their collective importance in maintaining skeletal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed : 15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization : while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity).

See full target information PHOSPHO1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Anais da Academia Brasileira de Ciencias 95:e20230480 PubMed38088732

2023

LINC00115 promotes gastric cancer partly by the miR-212-5p/ATPAF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxi Zhu,Jie Tan,Ting Zhan,Meng Liu,Yanli Zou,Weijie Liu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Phospho1 antibody

Western blot - Anti-Phospho1 antibody (AB101520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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