Skip to main content

Rabbit Polyclonal Phospho1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PHOSPHO1.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human PHOSPHO1. Database link Q8TCT1
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Polyclonal Phospho1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PHOSPHO1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human PHOSPHO1. Database link Q8TCT1
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purity >95%.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Phospho1 contributes to the mineralization process in bone and cartilage tissue. It plays an integral role in providing inorganic phosphate necessary for hydroxyapatite crystal formation an essential component of the mineral matrix. Phospho1 functions independently although its activity aligns with other enzymes and proteins involved in the mineralization process indicating its singular yet important enzymatic action in this biological context.

Activity summary

Phospho1 also known as PHOSPHO1 is a phosphatase with a molecular weight of approximately 32 kDa. It exhibits high phosphatase activity specific to phosphoethanolamine and phosphocholine. The enzyme localizes mainly in the ossification centers of developing bones particularly in osteoblasts and chondrocytes reflecting its significant role in skeletal mineralization.

Pathways

Phospho1 is directly involved in the skeletal development and bone mineralization pathways. It interacts closely within the matrix vesicle-mediated mineralization pathway. One notable related protein is alkaline phosphatase which cooperates to supply phosphate ions for matrix mineralization. This collaboration ensures effective hydroxyapatite crystal deposition thereby maintaining bone integrity and function.

Associated diseases and disorders

Phospho1 associates closely with conditions such as hypophosphatasia and osteoporosis. Its malfunction or dysregulation can cause impaired skeletal mineralization leading to these bone-related ailments. In the context of these conditions Phospho1 activity is linked to alkaline phosphatase as perturbation in either enzyme can result in altered bone mineral density and defective bone mineralization highlighting their collective importance in maintaining skeletal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (ab224724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (ab224724)

    Paraffin-embedded human heart tissue stained for Phospho1 using ab224724 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (ab224724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (ab224724)

    Paraffin-embedded human kidney tissue stained for Phospho1 using ab224724 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com