Rabbit Polyclonal Phospho1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PHOSPHO1 aa 150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PHOSPHO1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TCT1
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed:15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity).
Alternative names
Phosphoethanolamine/phosphocholine phosphatase, PHOSPHO1
Recommended products
Rabbit Polyclonal Phospho1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PHOSPHO1 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PHOSPHO1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TCT1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phospho1 also known as PHOSPHO1 is a phosphatase with a molecular weight of approximately 32 kDa. It exhibits high phosphatase activity specific to phosphoethanolamine and phosphocholine. The enzyme localizes mainly in the ossification centers of developing bones particularly in osteoblasts and chondrocytes reflecting its significant role in skeletal mineralization.
- Biological function summary
Phospho1 contributes to the mineralization process in bone and cartilage tissue. It plays an integral role in providing inorganic phosphate necessary for hydroxyapatite crystal formation an essential component of the mineral matrix. Phospho1 functions independently although its activity aligns with other enzymes and proteins involved in the mineralization process indicating its singular yet important enzymatic action in this biological context.
- Pathways
Phospho1 is directly involved in the skeletal development and bone mineralization pathways. It interacts closely within the matrix vesicle-mediated mineralization pathway. One notable related protein is alkaline phosphatase which cooperates to supply phosphate ions for matrix mineralization. This collaboration ensures effective hydroxyapatite crystal deposition thereby maintaining bone integrity and function.
- Associated diseases and disorders
Phospho1 associates closely with conditions such as hypophosphatasia and osteoporosis. Its malfunction or dysregulation can cause impaired skeletal mineralization leading to these bone-related ailments. In the context of these conditions Phospho1 activity is linked to alkaline phosphatase as perturbation in either enzyme can result in altered bone mineral density and defective bone mineralization highlighting their collective importance in maintaining skeletal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (ab272655)
Paraffin-embedded formalin-fixed human heart tissue stained for Phospho1 using ab272655 at 1/200 dilution in immunohistochemical analysis.
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