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AB272655

Anti-Phospho1 antibody

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Rabbit Polyclonal Phospho1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PHOSPHO1 aa 150-300.

View Alternative Names

Phosphoethanolamine/phosphocholine phosphatase, PHOSPHO1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (AB272655)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (AB272655)

Paraffin-embedded formalin-fixed human heart tissue stained for Phospho1 using ab272655 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PHOSPHO1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8TCT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospho1 also known as PHOSPHO1 is a phosphatase with a molecular weight of approximately 32 kDa. It exhibits high phosphatase activity specific to phosphoethanolamine and phosphocholine. The enzyme localizes mainly in the ossification centers of developing bones particularly in osteoblasts and chondrocytes reflecting its significant role in skeletal mineralization.
Biological function summary

Phospho1 contributes to the mineralization process in bone and cartilage tissue. It plays an integral role in providing inorganic phosphate necessary for hydroxyapatite crystal formation an essential component of the mineral matrix. Phospho1 functions independently although its activity aligns with other enzymes and proteins involved in the mineralization process indicating its singular yet important enzymatic action in this biological context.

Pathways

Phospho1 is directly involved in the skeletal development and bone mineralization pathways. It interacts closely within the matrix vesicle-mediated mineralization pathway. One notable related protein is alkaline phosphatase which cooperates to supply phosphate ions for matrix mineralization. This collaboration ensures effective hydroxyapatite crystal deposition thereby maintaining bone integrity and function.

Phospho1 associates closely with conditions such as hypophosphatasia and osteoporosis. Its malfunction or dysregulation can cause impaired skeletal mineralization leading to these bone-related ailments. In the context of these conditions Phospho1 activity is linked to alkaline phosphatase as perturbation in either enzyme can result in altered bone mineral density and defective bone mineralization highlighting their collective importance in maintaining skeletal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed : 15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization : while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity).
See full target information PHOSPHO1
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Product promise

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