AB219626

Anti-Phospholamban antibody [EPR21897]

Name: Anti-Phospholamban antibody [EPR21897] (ab219626) | Abcam
Brand: Abcam Limited
SKU: AB219626
Price: 355 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal Phospholamban antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

PLB, PLN, Phospholamban

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemical analysis of paraffin embedded human cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID : 28759816, PMID : 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Negative control : No staining shown in human tonsil tissue.

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (AB219626)

Phospholamban was immunoprecipitated from 0.35 mg of human fetal heart tissue lysate with ab219626 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219626 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human fetal heart tissue lysate 10 μg (Input).
Lane 2 : ab219626 IP in human fetal heart tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219626 in human fetal heart tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (ab219626)

Predicted band size: 6 kDa

Observed band size: 10 kDa

true

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemical analysis of paraffin embedded mouse cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse cardiac muscle (PMID : 28759816, PMID : 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemical analysis of paraffin embedded rat cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID : 28759816, PMID : 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-Phospholamban antibody [EPR21897] (AB219626)

Blocking and dilution buffer : 5% NFDM/TBST.

WB bands are consistent with reference (PMID 20696159; PMID 22144574)

All lanes:

Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626) at 1/2000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lanes 4 - 5:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 6 kDa

Observed band size: 10 kDa,25 kDa

true

Exposure time: 15s

Carrier free

Anti-Phospholamban antibody [EPR21897] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21897

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholamban abbreviated as PLN is an important regulatory protein in cardiac muscle cells often referred to interchangeably with its phosphorylated form phospho-phospholamban. This protein with a molecular mass of about 6 kDa is primarily expressed in cardiac and skeletal muscles. It functions mechanically by regulating the calcium pump (SERCA2a) in the sarcoplasmic reticulum modulating calcium uptake during muscle relaxation. In its unphosphorylated state phospholamban inhibits the activity of SERCA2a reducing calcium uptake and affecting muscle contractility.

Biological function summary

Phospholamban serves as an important mediator in the control of cardiac muscle contraction and relaxation. It is a component of the calcium cycling process within heart cells and associates directly with SERCA2a to form a regulatory complex. This association allows phospholamban to influence calcium homeostasis significantly affecting myocardial contractility and relaxation. Phosphorylation of phospholamban typically induced by beta-adrenergic signaling results in diminished interaction with SERCA2a enhancing calcium uptake into the sarcoplasmic reticulum.

Pathways

Several important pathways involve phospholamban including the adrenergic signaling pathway in cardiomyocytes and calcium signaling pathways. Phospholamban's role in these pathways is linked with proteins like SERCA2a and Protein Kinase A (PKA). PKA phosphorylates phospholamban an important step in the beta-adrenergic cascade that leads to increased heart muscle contractility. This phosphorylation event highlights phospholamban's participation in modulating cardiac output under sympathetic nervous system influence.

Phospholamban's regulation of calcium homeostasis connects it directly to conditions like heart failure and cardiomyopathy. In heart failure the dysregulation of phospholamban phosphorylation can lead to impaired cardiac function due to disrupted calcium cycling reducing cardiac output. Mutations in the phospholamban gene can lead to dilated cardiomyopathy a condition characterized by the enlargement and weakening of the heart muscle. These mutations affect the interaction with SERCA2a highlighting the role of phospholamban in maintaining cardiac muscle health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed : 28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity).

See full target information PLN

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113097 PubMed40822351

2025

SESN2 maintains cartilage homeostasis by SREBP1-mediated lipid metabolism during osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizheng Liu,Huiming Jiang,Zhaofeng Zhang,Ruiyang Jiang,Rongliang Wang,Jiawei Li,Weitong Li,Guihua Tan,Yuxiang Fei,Zhongyang Lv,Hu Guo,Ziyin Sun,Rui Wu,Hongfei Shi,Dongquan Shi

Cellular and molecular life sciences : CMLS 82:50 PubMed39833543

2025

Single-cell RNA sequencing of the carotid artery and femoral artery of rats exposed to hindlimb unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfei Li,Yikai Pan,Yuan Wang,Xi Li,Yateng Tie,Shuhan Li,Ruonan Wang,Xingcheng Zhao,Jieyi Fan,Xianchun Yan,Yongchun Wang,Xiqing Sun

International journal of molecular medicine 55: PubMed39821325

2025

Protective role of triiodothyronine in sepsis‑induced cardiomyopathy through phospholamban downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiumin Xie,Qin Yi,Jing Zhu,Bin Tan,Han Xiang,Rui Wang,Huiwen Liu,Tangtian Chen,Hao Xu

Molecular and cellular biology 44:149-163 PubMed38725392

2024

Effects of Phenylacetylglutamine on the Susceptibility of Atrial Fibrillation in Overpressure-Induced HF Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Fu,Dengke Li,Wei Shuai,Bin Kong,Xi Wang,Yanhong Tang,He Huang,Congxin Huang

GeroScience 46:4543-4561 PubMed38656649

2024

Sex-specific cardiovascular remodeling leads to a divergent sex-dependent development of heart failure in aged hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Árpád Kovács,Saltanat Zhazykbayeva,Melissa Herwig,Gábor Á Fülöp,Tamás Csípő,Nikolett Oláh,Roua Hassoun,Heidi Budde,Hersh Osman,Mustafa Kaçmaz,Kornelia Jaquet,Dániel Priksz,Béla Juhász,Ibrahim Akin,Zoltán Papp,Wolfgang E Schmidt,Andreas Mügge,Ibrahim El-Battrawy,Attila Tóth,Nazha Hamdani

Cellular and molecular life sciences : CMLS 81:88 PubMed38349408

2024

Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Xing Wei,Xing-Xing Cai,Yu-Dong Fei,Qian Wang,Xiao-Liang Hu,Cheng Li,Jian-Wen Hou,Yu-Li Yang,Tai-Zhong Chen,Xiao-Lei Xu,Yue-Peng Wang,Yi-Gang Li

American journal of physiology. Heart and circulatory physiology 326:H870-H876 PubMed38334971

2024

Deletion of DWORF does not affect cardiac function in aging and in PLN-R14del cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nienke M Stege,Vivian Oliveira Nunes Teixeira,Sietske N Zijlstra,Anna M Feringa,Rudolf A de Boer,Herman H W Silljé

Nature cardiovascular research 1:577-591 PubMed39195867

2022

Piezo1 is the cardiac mechanosensor that initiates the cardiomyocyte hypertrophic response to pressure overload in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Yan Yu,Hutao Gong,Scott Kesteven,Yang Guo,Jianxin Wu,Jinyuan Vero Li,Delfine Cheng,Zijing Zhou,Siiri E Iismaa,Xenia Kaidonis,Robert M Graham,Charles D Cox,Michael P Feneley,Boris Martinac

Aging and disease 13:298-312 PubMed35111375

2022

Atrial Fibrillation Underlies Cardiomyocyte Senescence and Contributes to Deleterious Atrial Remodeling during Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ailiya Adili,Xiyu Zhu,Hailong Cao,Xinlong Tang,Yali Wang,Junxia Wang,Jian Shi,Qing Zhou,Dongjin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholamban antibody [EPR21897]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

Western blot - Anti-Phospholamban antibody [EPR21897] (AB219626)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise