AB92697

Anti-Phospholamban (phospho S16) antibody [EPR1911]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Phospholamban phospho S16 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PLB, PLN, Phospholamban

1 Images

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Western blot - Anti-Phospholamban (phospho S16) antibody [EPR1911] (AB92697)

Actual band size is 11 KDa.

All lanes:

Western blot - Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution

Lane 1:

Fetal heart lysate (untreated) at 10 µg

Lane 2:

Fetal heart lysate (treated with LP) at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Predicted band size: 6 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1911

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000 - 1/500000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

This antibody is not purified. It is provided in cell supernatant and storage buffer.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholamban abbreviated as PLN is an important regulatory protein in cardiac muscle cells often referred to interchangeably with its phosphorylated form phospho-phospholamban. This protein with a molecular mass of about 6 kDa is primarily expressed in cardiac and skeletal muscles. It functions mechanically by regulating the calcium pump (SERCA2a) in the sarcoplasmic reticulum modulating calcium uptake during muscle relaxation. In its unphosphorylated state phospholamban inhibits the activity of SERCA2a reducing calcium uptake and affecting muscle contractility.

Biological function summary

Phospholamban serves as an important mediator in the control of cardiac muscle contraction and relaxation. It is a component of the calcium cycling process within heart cells and associates directly with SERCA2a to form a regulatory complex. This association allows phospholamban to influence calcium homeostasis significantly affecting myocardial contractility and relaxation. Phosphorylation of phospholamban typically induced by beta-adrenergic signaling results in diminished interaction with SERCA2a enhancing calcium uptake into the sarcoplasmic reticulum.

Pathways

Several important pathways involve phospholamban including the adrenergic signaling pathway in cardiomyocytes and calcium signaling pathways. Phospholamban's role in these pathways is linked with proteins like SERCA2a and Protein Kinase A (PKA). PKA phosphorylates phospholamban an important step in the beta-adrenergic cascade that leads to increased heart muscle contractility. This phosphorylation event highlights phospholamban's participation in modulating cardiac output under sympathetic nervous system influence.

Phospholamban's regulation of calcium homeostasis connects it directly to conditions like heart failure and cardiomyopathy. In heart failure the dysregulation of phospholamban phosphorylation can lead to impaired cardiac function due to disrupted calcium cycling reducing cardiac output. Mutations in the phospholamban gene can lead to dilated cardiomyopathy a condition characterized by the enlargement and weakening of the heart muscle. These mutations affect the interaction with SERCA2a highlighting the role of phospholamban in maintaining cardiac muscle health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed : 28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity).

See full target information PLN phospho S16

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acupuncture in medicine : journal of the British Medical Acupuncture Society 42:342-355 PubMed39579035

2024

Electroacupuncture alleviates acute myocardial ischemic injury in mice by regulating the β adrenergic receptor and post-receptor protein kinase A signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Zuo,Qiaoyu Qu,Yan Tong,Lei Wang,Xiaoxiao Wang,Shengbing Wu,Meiqi Zhou

Cell reports methods 3:100456 PubMed37159667

2023

Generation of left ventricle-like cardiomyocytes with improved structural, functional, and metabolic maturity from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Dark,Marie-Victoire Cosson,Lorenza I Tsansizi,Thomas J Owen,Elisa Ferraro,Alice J Francis,Selina Tsai,Camille Bouissou,Anne Weston,Lucy Collinson,Najah Abi-Gerges,Paul E Miller,Kenneth T MacLeod,Elisabeth Ehler,Richard Mitter,Sian E Harding,James C Smith,Andreia S Bernardo

Molecular and cellular biochemistry 476:3079-3087 PubMed33811579

2021

Pleiotropic, non-cell death-associated effects of inhibitors of receptor-interacting protein kinase 1 in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

C Horvath,A Szobi,L Kindernay,T Ravingerova,A Adameova

Frontiers in physiology 9:1383 PubMed30356673

2018

Profibrotic, Electrical, and Calcium-Handling Remodeling of the Atria in Heart Failure Patients With and Without Atrial Fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina E Molina,Issam H Abu-Taha,Qiongling Wang,Elena Roselló-Díez,Marcus Kamler,Stanley Nattel,Ursula Ravens,Xander H T Wehrens,Leif Hove-Madsen,Jordi Heijman,Dobromir Dobrev

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholamban (phospho S16) antibody [EPR1911]

Western blot - Anti-Phospholamban (phospho S16) antibody [EPR1911] (AB92697)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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Product promise