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AB62170

Anti-Phospholamban (phospho S16 + T17) antibody

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(7 Publications)

Rabbit Polyclonal Phospholamban phospho S16 + T17 antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human PLN pS16 + T17.

View Alternative Names

PLB, PLN, Phospholamban, pln thr17, pln ser16

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Phospholamban (phospho S16 + T17) antibody (AB62170)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Phospholamban (phospho S16 + T17) antibody (AB62170)

Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human PLN pS16 + T17. The exact immunogen used to generate this antibody is proprietary information.

P26678

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholamban abbreviated as PLN is an important regulatory protein in cardiac muscle cells often referred to interchangeably with its phosphorylated form phospho-phospholamban. This protein with a molecular mass of about 6 kDa is primarily expressed in cardiac and skeletal muscles. It functions mechanically by regulating the calcium pump (SERCA2a) in the sarcoplasmic reticulum modulating calcium uptake during muscle relaxation. In its unphosphorylated state phospholamban inhibits the activity of SERCA2a reducing calcium uptake and affecting muscle contractility.
Biological function summary

Phospholamban serves as an important mediator in the control of cardiac muscle contraction and relaxation. It is a component of the calcium cycling process within heart cells and associates directly with SERCA2a to form a regulatory complex. This association allows phospholamban to influence calcium homeostasis significantly affecting myocardial contractility and relaxation. Phosphorylation of phospholamban typically induced by beta-adrenergic signaling results in diminished interaction with SERCA2a enhancing calcium uptake into the sarcoplasmic reticulum.

Pathways

Several important pathways involve phospholamban including the adrenergic signaling pathway in cardiomyocytes and calcium signaling pathways. Phospholamban's role in these pathways is linked with proteins like SERCA2a and Protein Kinase A (PKA). PKA phosphorylates phospholamban an important step in the beta-adrenergic cascade that leads to increased heart muscle contractility. This phosphorylation event highlights phospholamban's participation in modulating cardiac output under sympathetic nervous system influence.

Phospholamban's regulation of calcium homeostasis connects it directly to conditions like heart failure and cardiomyopathy. In heart failure the dysregulation of phospholamban phosphorylation can lead to impaired cardiac function due to disrupted calcium cycling reducing cardiac output. Mutations in the phospholamban gene can lead to dilated cardiomyopathy a condition characterized by the enlargement and weakening of the heart muscle. These mutations affect the interaction with SERCA2a highlighting the role of phospholamban in maintaining cardiac muscle health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed : 28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity).
See full target information PLN pS16 + T17
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed41007781

2025

Prior Aerobic Exercise Training Fails to Confer Cardioprotection Under Varying Exercise Volumes in Early Post-Infarction Cardiac Remodeling in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

André Rodrigues Lourenço Dias,Ednei Luiz Antonio,Helenita Antonia de Oliveira,Ighor Luiz Azevedo Teixeira,Larissa Emília Seibt,Andrey Jorge Serra

BMC cardiovascular disorders 24:197 PubMed38580957

2024

HAPLN1 knockdown inhibits heart failure development via activating the PKA signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yan,Shushuai Song,Wendong Sun,Yiping Ge

Redox biology 44:102016 PubMed34038814

2021

Histidine dipeptides are key regulators of excitation-contraction coupling in cardiac muscle: Evidence from a novel CARNS1 knockout rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Lívia de Souza Gonçalves,Lucas Peixoto Sales,Tiemi Raquel Saito,Juliane Cruz Campos,Alan Lins Fernandes,José Natali,Leonardo Jensen,Alexandre Arnold,Lisley Ramalho,Luiz Roberto Grassmann Bechara,Marcos Vinicius Esteca,Isis Correa,Diogo Sant'Anna,Alexandre Ceroni,Lisete Compagno Michelini,Bruno Gualano,Walcy Teodoro,Victor Henrique Carvalho,Bianca Scigliano Vargas,Marisa Helena Gennari Medeiros,Igor Luchini Baptista,Maria Cláudia Irigoyen,Craig Sale,Julio Cesar Batista Ferreira,Guilherme Giannini Artioli

Experimental and therapeutic medicine 18:4631-4636 PubMed31798700

2019

Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Wei Li,Lirong Wu,Long Wang,Yongkang Li,Wei Zhou

Physiological reports 7:e13972 PubMed30806037

2019

Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rujie Qin,Nobuyuki Murakoshi,DongZhu Xu,Kazuko Tajiri,Duo Feng,Endin N Stujanna,Saori Yonebayashi,Yoshimi Nakagawa,Hitoshi Shimano,Akihiko Nogami,Akira Koike,Kazutaka Aonuma,Masaki Ieda

Cardiovascular research 108:197-208 PubMed26378152

2015

LKB1 deletion causes early changes in atrial channel expression and electrophysiology prior to atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Grace E Kim,Jenna L Ross,Chaoqin Xie,Kevin N Su,Vlad G Zaha,Xiaohong Wu,Monica Palmeri,Mohammed Ashraf,Joseph G Akar,Kerry S Russell,Fadi G Akar,Lawrence H Young

PloS one 8:e82552 PubMed24386101

2013

Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation.

Applications

WB

Species

Rat

Yu Huang,Qing He,Lei Zhan,Min Yang
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