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AB308132

Anti-Phospholamban (phospho S16 + T17) antibody [RP23040185]

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Rabbit Recombinant Multiclonal Phospholamban phospho S16 + T17 antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human PLN phospho S16 + T17.

View Alternative Names

PLB, PLN, Phospholamban

1 Images
Western blot - Anti-Phospholamban (phospho S16 + T17) antibody [RP23040185] (AB308132)
  • WB

Supplier Data

Western blot - Anti-Phospholamban (phospho S16 + T17) antibody [RP23040185] (AB308132)

Western blot analysis was performed on Tissue extracts (30 µg lysate) of Rat Brain (Lane 1) and Mouse Brain (Lane 2). The blots were probed with Phospho-Phospholamban (Ser16, Thr17) Antibody, Recombinant Rabbit Polyclonal Antibody (ab308132, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 06 kDa band corresponding to Phospho-Phospholamban (Ser16, Thr17) was observed. To confirm the specificity of Phospho-Phospholamban (Ser16, Thr17) Antibody, Recombinant Rabbit Polyclonal Antibody, competition was performed with the posphopeptide (10 µg/mL) as shown in the corresponding blot on the right. The phosphopeptide competes with the antibody and prevents it from binding to the target protein. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary.

All lanes:

Western blot - Anti-Phospholamban (phospho S16 + T17) antibody [RP23040185] (ab308132) at 1 µg/mL

Lane 1:

Rat brain tissue lysate at 30 µg

Lane 2:

Mouse brain tissue lysate at 30 µg

Lane 3:

Rat brain tissue lysate treated with phosphopeptide at 30 µg

Lane 4:

Mouse brain tissue lysate treated with phosphopeptide at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 6 kDa

Observed band size: 6 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040185

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PLN phospho S16 + T17.

P26678

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholamban abbreviated as PLN is an important regulatory protein in cardiac muscle cells often referred to interchangeably with its phosphorylated form phospho-phospholamban. This protein with a molecular mass of about 6 kDa is primarily expressed in cardiac and skeletal muscles. It functions mechanically by regulating the calcium pump (SERCA2a) in the sarcoplasmic reticulum modulating calcium uptake during muscle relaxation. In its unphosphorylated state phospholamban inhibits the activity of SERCA2a reducing calcium uptake and affecting muscle contractility.
Biological function summary

Phospholamban serves as an important mediator in the control of cardiac muscle contraction and relaxation. It is a component of the calcium cycling process within heart cells and associates directly with SERCA2a to form a regulatory complex. This association allows phospholamban to influence calcium homeostasis significantly affecting myocardial contractility and relaxation. Phosphorylation of phospholamban typically induced by beta-adrenergic signaling results in diminished interaction with SERCA2a enhancing calcium uptake into the sarcoplasmic reticulum.

Pathways

Several important pathways involve phospholamban including the adrenergic signaling pathway in cardiomyocytes and calcium signaling pathways. Phospholamban's role in these pathways is linked with proteins like SERCA2a and Protein Kinase A (PKA). PKA phosphorylates phospholamban an important step in the beta-adrenergic cascade that leads to increased heart muscle contractility. This phosphorylation event highlights phospholamban's participation in modulating cardiac output under sympathetic nervous system influence.

Phospholamban's regulation of calcium homeostasis connects it directly to conditions like heart failure and cardiomyopathy. In heart failure the dysregulation of phospholamban phosphorylation can lead to impaired cardiac function due to disrupted calcium cycling reducing cardiac output. Mutations in the phospholamban gene can lead to dilated cardiomyopathy a condition characterized by the enlargement and weakening of the heart muscle. These mutations affect the interaction with SERCA2a highlighting the role of phospholamban in maintaining cardiac muscle health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed : 28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity).
See full target information PLN phospho S16 + T17
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