AB133589

Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal Phospholipase A2 activator protein (PLAP) antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PLAP, PLAA, Phospholipase A-2-activating protein, PLA2P

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Phospholipase A2 activator protein (PLAP) with ab133589 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

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Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 87 kDa

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Lab

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

Lanes 1-4 : Merged signal (red and green). Green - ab133589 observed at 87 kDa. Red - loading control ab8245 observed at 37 kDa.

ab133589 Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] was shown to specifically react with PLAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265255 (knockout cell lysate ab258121) was used. Wild-type and PLAP knockout samples were subjected to SDS-PAGE. ab133589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PLAP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PLAA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plaa-knockout-hela-cell-line-ab265255'>ab265255</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

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OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

OI-RD Scanning

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OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5481

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase A2 activator protein (PLAP) also known as phospholipase A facilitates the activation of phospholipase A2 enzymes. PLAP assists in the release of arachidonic acid from phospholipids within cellular membranes. The protein is approximately 30 kDa in mass and expresses widely in various tissues including liver and brain. Researchers frequently study PLAP to understand its role in modulating lipid signaling pathways.

Biological function summary

This protein actively participates in cellular responses by modulating the production of lipid mediators. PLAP does not work standalone; it forms part of the calcium-dependent phospholipid-binding complex. Through its role in this complex it influences numerous processes such as inflammation and immune response. PLAP is also essential for the optimal function of inflammatory cell membranes by regulating the release of pro-inflammatory eicosanoids.

Pathways

This protein contributes significantly to the arachidonic acid metabolism pathway and eicosanoid biosynthesis pathway. In these pathways PLAP ensures the effectiveness of phospholipase A2 enabling the release of arachidonic acid a precursor for prostaglandins and leukotrienes. These components influence key activities in cellular signaling. Proteins such as cyclooxygenase (COX) and lipoxygenase follow through these pathways affecting cellular responses.

Alterations in PLAP activity relate to inflammatory conditions such as asthma and arthritis. The protein's role in the overproduction of eicosanoids connects it to the inflammation seen in these diseases. Another protein cyclooxygenase-2 (COX-2) also plays a significant part in these conditions acting downstream in the arachidonic acid pathway to produce inflammatory mediators. This link makes PLAP a significant target for therapeutic intervention in managing inflammation-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in protein ubiquitination, sorting and degradation through its association with VCP (PubMed : 27753622). Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling (By similarity). May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed : 27753622). Plays a role in cerebellar Purkinje cell development (By similarity). Positively regulates cytosolic and calcium-independent phospholipase A2 activities in a tumor necrosis factor alpha (TNF-alpha)- or lipopolysaccharide (LPS)-dependent manner, and hence prostaglandin E2 biosynthesis (PubMed : 18291623, PubMed : 28007986).

See full target information PLAA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:1562-1581 PubMed36300783

2022

PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Bai,Guimei Yu,Hong-Ming Zhou,Ovini Amarasinghe,Yuan Zhou,Peipei Zhu,Qinglin Li,Lujuan Zhang,Frederick Nguele Meke,Yiming Miao,Eli Chapman,W Andy Tao,Zhong-Yin Zhang

Scientific reports 11:10981 PubMed34040082

2021

Analysis of the intracellular traffic of IgG in the context of Down syndrome (trisomy 21).

Applications

Unspecified application

Species

Unspecified reactive species

R B Cejas,M Tamaño-Blanco,J G Blanco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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