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AB248568

Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] - BSA and Azide free

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Rabbit Recombinant Monoclonal Phospholipase A2 activator protein (PLAP) antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

PLAP, PLAA, Phospholipase A-2-activating protein, PLA2P

1 Images
Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] - BSA and Azide free (AB248568)
  • WB

Lab

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] - BSA and Azide free (AB248568)

This data was developed using the same antibody clone in a different buffer formulation (ab133589).

Lanes 1-4 : Merged signal (red and green). Green - ab133589 observed at 87 kDa. Red - loading control ab8245 observed at 37 kDa.

ab133589 Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] was shown to specifically react with PLAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265255 (knockout cell lysate ab258121) was used. Wild-type and PLAP knockout samples were subjected to SDS-PAGE. ab133589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (<a href='/en-us/products/primary-antibodies/phospholipase-a2-activator-protein-plap-antibody-epr5481-ab133589'>ab133589</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PLAP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PLAA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plaa-knockout-hela-cell-line-ab265255'>ab265255</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

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  • Unconjugated

    Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5481

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Product details

ab248568 is the carrier-free version of ab133589.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase A2 activator protein (PLAP) also known as phospholipase A facilitates the activation of phospholipase A2 enzymes. PLAP assists in the release of arachidonic acid from phospholipids within cellular membranes. The protein is approximately 30 kDa in mass and expresses widely in various tissues including liver and brain. Researchers frequently study PLAP to understand its role in modulating lipid signaling pathways.
Biological function summary

This protein actively participates in cellular responses by modulating the production of lipid mediators. PLAP does not work standalone; it forms part of the calcium-dependent phospholipid-binding complex. Through its role in this complex it influences numerous processes such as inflammation and immune response. PLAP is also essential for the optimal function of inflammatory cell membranes by regulating the release of pro-inflammatory eicosanoids.

Pathways

This protein contributes significantly to the arachidonic acid metabolism pathway and eicosanoid biosynthesis pathway. In these pathways PLAP ensures the effectiveness of phospholipase A2 enabling the release of arachidonic acid a precursor for prostaglandins and leukotrienes. These components influence key activities in cellular signaling. Proteins such as cyclooxygenase (COX) and lipoxygenase follow through these pathways affecting cellular responses.

Alterations in PLAP activity relate to inflammatory conditions such as asthma and arthritis. The protein's role in the overproduction of eicosanoids connects it to the inflammation seen in these diseases. Another protein cyclooxygenase-2 (COX-2) also plays a significant part in these conditions acting downstream in the arachidonic acid pathway to produce inflammatory mediators. This link makes PLAP a significant target for therapeutic intervention in managing inflammation-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in protein ubiquitination, sorting and degradation through its association with VCP (PubMed : 27753622). Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling (By similarity). May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed : 27753622). Plays a role in cerebellar Purkinje cell development (By similarity). Positively regulates cytosolic and calcium-independent phospholipase A2 activities in a tumor necrosis factor alpha (TNF-alpha)- or lipopolysaccharide (LPS)-dependent manner, and hence prostaglandin E2 biosynthesis (PubMed : 18291623, PubMed : 28007986).
See full target information PLAA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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