Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] - BSA and Azide free (AB247246)

This data was developed using ab32354, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Phospholipase A2 activator protein (PLAP) with ab32354 at 1/2000 dilution.

The primary antibody was incubated for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] - BSA and Azide free (AB247246)

This data was developed using ab32354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (<a href='/en-us/products/primary-antibodies/phospholipase-a2-activator-protein-plap-antibody-y102-ab32354'>ab32354</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

true

Exposure time: 10s