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AB243181

Anti-Phospholipase C antibody [bc-01]

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(3 Publications)

Mouse Monoclonal PHLC antibody. Suitable for WB, ELISA and reacts with Bacillus cereus, Purified native protein - Bacillus cereus samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Bacillus cereus protein.

View Alternative Names

Phospholipase C, PLC, Cereolysin A, Phosphatidylcholine cholinephosphohydrolase, plc

3 Images
ELISA - Anti-Phospholipase C antibody [bc-01] (AB243181)
  • ELISA

Supplier Data

ELISA - Anti-Phospholipase C antibody [bc-01] (AB243181)

Titration of PLC in the extract of B. cereus cells by indirect ELISA using ab243181.

An ELISA plate was coated with indicated amounts of the extract of B. cereus cells per well.

ab243181 was used at a 1/500 dilution.

ELISA - Anti-Phospholipase C antibody [bc-01] (AB243181)
  • ELISA

Supplier Data

ELISA - Anti-Phospholipase C antibody [bc-01] (AB243181)

Titration ab243181 by indirect ELISA using crude extract of B. cereus.

An ELISA plate was coated with crude extract of B. cereus (100 μL, 1 μg/mL). After blocking with 5% skimmed milk, 100 μL of antibody at the indicated dilutions was added to each well. HRP-conjugated goat anti-mouse IgM (100 μL, X2000 dilution) was added.

Western blot - Anti-Phospholipase C antibody [bc-01] (AB243181)
  • WB

Supplier Data

Western blot - Anti-Phospholipase C antibody [bc-01] (AB243181)

All lanes:

Western blot - Anti-Phospholipase C antibody [bc-01] (ab243181) at 1/1000 dilution

Lane 1:

Culture medium of B. cereus

Lane 2:

PC-PLC purified from B. cereus

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

bc-01

Isotype

IgM

Carrier free

No

Reacts with

Bacillus cereus

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with phosphatidylcholine phospholipase C (PC-PLC) of Bacillus cereus.

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Secondary Antibodies

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Primary Antibodies

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  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacillus cereus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Purified native protein - Bacillus cereus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C also known as PLC is an enzyme that catalyzes the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3). It has several isoforms such as PLC beta and PLC gamma each with different molecular sizes. For example PLC gamma typically has a mass of about 130 kDa. This enzyme is expressed in many tissues including the brain liver and pancreas where it plays an important role in cellular signaling.
Biological function summary

The activity of Phospholipase C generates second messengers that are important for intracellular signal transduction. It functions as part of larger signaling complexes and can be involved in regulating various cellular processes like proliferation differentiation and apoptosis. This role makes it an important regulator in many physiological responses. The enzyme often interacts with membrane-bound receptors linking extracellular signals to internal cellular responses.

Pathways

Phospholipase C is an important player in the phosphatidylinositol signaling pathway and is interconnected with the calcium signaling pathway. Within these pathways PLC activity triggers the release of calcium from internal stores which in turn can activate calcium-dependent proteins such as protein kinase C. These interactions aid in modulating cellular responses to external stimuli.

PLC abnormalities have been linked to conditions like cancer and bipolar disorder. Altered PLC function can disrupt normal cellular signaling contributing to the pathogenesis of these diseases. In cancer abnormal PLC activity is tied to uncontrolled cell growth often interacting with proteins like Ras to drive oncogenic processes. In bipolar disorder disruptions in PLC function affect neurotransmitter pathways implicating changes in proteins associated with synaptic signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
See full target information plc
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32455738

2020

Interstitial Leydig Cell Tumorigenesis-Leptin and Adiponectin Signaling in Relation to Aromatase Expression in the Human Testis.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Duliban,Ewelina Gorowska-Wojtowicz,Waclaw Tworzydlo,Agnieszka Rak,Malgorzata Brzoskwinia,Izabella Krakowska,Jan K Wolski,Malgorzata Kotula-Balak,Bartosz J Płachno,Barbara Bilinska

Journal of diabetes research 2019:7602427 PubMed31950065

2019

Metformin Reduces Lipotoxicity-Induced Meta-Inflammation in -Cells through the Activation of GPR40-PLC-IP3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ximei Shen,Beibei Fan,Xin Hu,Liufen Luo,Yuanli Yan,Liyong Yang

Journal of experimental botany 64:3169-78 PubMed23788722

2013

Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Leonor Ramírez,Carlos Guillermo Bartoli,Lorenzo Lamattina
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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