Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
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(9 Publications)
Rabbit Recombinant Monoclonal Phospholipase C beta 1/PLCB1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
View Alternative Names
KIAA0581, PLCB1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, Phospholipase C-beta-1, PLC-I, PLC-beta-1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on normal Human cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human gastric cancer. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on rat cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded frozen, 0.2% Triton X-100 permeabilized Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).
The result showed cytoplasmic staining on mouse cerebrum.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Blocking/Dilution buffer : 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 8429045, 2468162).
All lanes:
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1:
Human brain lysate at 20 µg
Lane 2:
HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 100 kDa,150 kDa
false
Exposure time: 3min
- WB
Unknown
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Lanes 1-4 : Merged signal (red and green). Green - ab182359 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.
ab182359 Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] was shown to specifically react with Phospholipase C beta 1/PLCB1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266033 (knockout cell lysate ab257589) was used. Wild-type and Phospholipase C beta 1/PLCB1 knockout samples were subjected to SDS-PAGE. ab182359 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PLCB1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human Plcb1 (Phospholipase C beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plcb1-phospholipase-c-beta-1-knockout-hela-cell-line-ab266033'>ab266033</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
Mouse heart tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa
false
- WB
Supplier Data
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Blocking/Dilution buffer : 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8429045, 2468162).
All lanes:
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1:
Mouse brain lysate at 10 µg
Lane 2:
Mouse heart lysate at 10 µg
Lane 3:
Mouse spleen lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 139 kDa
Observed band size: 100 kDa,150 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Blocking/Dilution buffer : 5% NFDM/TBST.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 8429045, 2468162).
All lanes:
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1:
Rat brain lysate at 10 µg
Lane 2:
Rat heart lysate at 10 µg
Lane 3:
Rat spleen lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 139 kDa
Observed band size: 100 kDa,150 kDa
false
Exposure time: 1s
- WB
Supplier Data
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (AB182359)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1 : 3minutes; Lane 2 and 3 : 10 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 8429045, 2468162).
All lanes:
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1:
NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 2:
C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 3:
PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 139 kDa
Observed band size: 100 kDa,150 kDa
false
Related conjugates and formulations (1)
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Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Multiple functions depend on the signaling cascades initiated by PLCB1. It belongs to a larger family of phospholipase C enzymes contributing to the regulation of various neural processes. The enzyme acts within multiprotein complexes that modulate intracellular calcium levels influencing neuronal activity and synaptic transmission. This role implicates PLCB1 in modulating brain functions related to learning and memory.
Pathways
PLCB1 contributes to cellular signaling pathways such as the phosphoinositide signaling pathway and the G protein-coupled receptor (GPCR) pathway. In these pathways PLCB1 interacts with major proteins such as Gαq subunit of G proteins which activates it. These interactions place PLCB1 as a central player in the pathways that help convert extracellular signals into intracellular actions often involving the regulation of secondary messengers like IP3 and DAG.
Product protocols
- Visit the General protocols
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Target data
Publications (9)
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Chemistry & biodiversity 22:e202402771 PubMed39776239
2025
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Molecular carcinogenesis 64:162-175 PubMed39451071
2024
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Cancer science 115:197-210 PubMed37882467
2023
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Frontiers in molecular neuroscience 16:1118707 PubMed37063368
2023
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Cancer cell international 21:587 PubMed34727945
2021
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Acta physiologica (Oxford, England) 232:e13693 PubMed34028994
2021
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Digestive diseases and sciences : PubMed33559088
2021
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Cell reports 27:2636-2648.e4 PubMed31141688
2019
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The Journal of pharmacology and experimental thera 369:244-258 PubMed30867225
2019
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Product promise
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