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AB240260

Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Phospholipase C beta 1/PLCB1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA0581, PLCB1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, Phospholipase C-beta-1, PLC-I, PLC-beta-1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on normal Human cerebrum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human gastric cancer. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat cerebrum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse hippocampus is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

Immunohistochemical analysis of paraffin-embedded frozen, 0.2% Triton X-100 permeabilized Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse cerebrum.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)
  • WB

Unknown

Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (AB240260)

This data was developed using the same antibody clone in a different buffer formulation (ab182359).

Lanes 1-4 : Merged signal (red and green). Green - ab182359 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182359 Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] was shown to specifically react with Phospholipase C beta 1/PLCB1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266033 (knockout cell lysate ab257589) was used. Wild-type and Phospholipase C beta 1/PLCB1 knockout samples were subjected to SDS-PAGE. ab182359 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (<a href='/en-us/products/primary-antibodies/phospholipase-c-beta-1-plcb1-antibody-epr19085-ab182359'>ab182359</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PLCB1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human Plcb1 (Phospholipase C beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plcb1-phospholipase-c-beta-1-knockout-hela-cell-line-ab266033'>ab266033</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

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  • Unconjugated

    Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19085

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB302940

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma]

Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma] (AB302940)

  • 0
  • 0
Primary Antibodies

AB184743

Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB108517

Anti-IP3 receptor antibody [EPR4537]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (AB108517)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Perform antigen retrieval with heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab240260 is the carrier-free version of ab182359.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C beta 1 (PLCB1) also known as PLC-β1 or PLCB1 is an enzyme involved in intracellular signal transduction. With a molecular weight of around 150 kDa PLCB1 hydrolyzes phosphatidylinositol 45-bisphosphate (PIP2) into inositol trisphosphate (IP3) and diacylglycerol (DAG). This biochemical activity plays an important role in the release of calcium ions from intracellular stores. PLCB1 is expressed in regions of the brain including the cerebral cortex as well as in other tissues.
Biological function summary

Multiple functions depend on the signaling cascades initiated by PLCB1. It belongs to a larger family of phospholipase C enzymes contributing to the regulation of various neural processes. The enzyme acts within multiprotein complexes that modulate intracellular calcium levels influencing neuronal activity and synaptic transmission. This role implicates PLCB1 in modulating brain functions related to learning and memory.

Pathways

PLCB1 contributes to cellular signaling pathways such as the phosphoinositide signaling pathway and the G protein-coupled receptor (GPCR) pathway. In these pathways PLCB1 interacts with major proteins such as Gαq subunit of G proteins which activates it. These interactions place PLCB1 as a central player in the pathways that help convert extracellular signals into intracellular actions often involving the regulation of secondary messengers like IP3 and DAG.

Researchers associate PLCB1 with neurological and psychiatric conditions including epilepsy and bipolar disorder. Dysregulation in PLCB1 activity links to altered calcium signaling which can affect brain function. Additionally proteins like Gαq and other GPCR pathway elements may either buffer or exacerbate effects arising from PLCB1 anomalies suggesting molecular interdependencies in related disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors (PubMed : 9188725). Regulates the function of the endothelial barrier.
See full target information PLCB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:3120-3140.e29 PubMed38714197

2024

Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Shovik Bandyopadhyay,Michael P Duffy,Kyung Jin Ahn,Jonathan H Sussman,Minxing Pang,David Smith,Gwendolyn Duncan,Iris Zhang,Jeffrey Huang,Yulieh Lin,Barbara Xiong,Tamjid Imtiaz,Chia-Hui Chen,Anusha Thadi,Changya Chen,Jason Xu,Melissa Reichart,Zachary Martinez,Caroline Diorio,Chider Chen,Vinodh Pillai,Oraine Snaith,Derek Oldridge,Siddharth Bhattacharyya,Ivan Maillard,Martin Carroll,Charles Nelson,Ling Qin,Kai Tan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com