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Rabbit Recombinant Monoclonal Phospholipase C beta 3/PLCB3 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743), expandable thumbnail
  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743), expandable thumbnail
  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743), expandable thumbnail
  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Expected
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/20

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Targets

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1

Alternative names

Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3, PLC-beta-3, PLCB3

Recommended products

Rabbit Recombinant Monoclonal Phospholipase C beta 3/PLCB3 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18714

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Phospholipase C beta 1 (PLCB1) and Phospholipase C beta 3 (PLCB3) are enzymes known for their role in phosphoinositide hydrolysis. These proteins catalyze the conversion of phosphatidylinositol 45-bisphosphate (PIP2) into inositol trisphosphate (IP3) and diacylglycerol (DAG) important secondary messengers in cellular signaling. PLCB1 has a molecular mass of approximately 138 kDa while PLCB3 is approximately 150 kDa. Both PLCB1 and PLCB3 are expressed widely in the central nervous system and various peripheral tissues indicating their involvement in diverse physiological processes.

Biological function summary

PLCB1 and PLCB3 influence intracellular calcium levels and protein kinase C activity by generating IP3 and DAG respectively. These proteins do not operate in isolation but interact with other signaling molecules forming parts of larger signaling complexes. They modulate neuronal signaling pathways which impact processes such as neurotransmitter release. This involvement highlights their importance in maintaining normal cellular communication and function.

Pathways

PLCB1 and PLCB3 play significant roles in the G-protein coupled receptor (GPCR) signaling pathway. They function downstream of the receptor activation being activated by Gq proteins leading to the production of secondary messengers. The enzymes also participate in the phosphatidylinositol signaling pathway particularly influencing calcium signaling. The interaction of PLCB1 and PLCB3 with Gq proteins highlights their integration into diverse signaling networks that communicate extracellular signals to elicit intracellular responses.

Associated diseases and disorders

Dysfunction of PLCB1 and PLCB3 links to various neurological diseases and certain forms of cancer. Abnormalities in PLCB1 expression associate with epilepsy indicating its role in neural excitability and signaling balance. Altered PLCB3 activity contributes to the development of breast cancer where its interaction with proteins like protein kinase C influences proliferative signaling pathways. These connections highlight the potential of targeting PLCB1 and PLCB3 to develop therapeutic strategies for related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human Phospholipase C beta 1 recombinant protein fragment contains aa518-656 with a His-Tag®. Human Phospholipase C beta 3 recombinant protein fragment contains aa590-706 with a His-Tag®. The immunogen of this product has low homology with Phospholipase C beta 2 and Phospholipase C beta 4.

    All lanes: Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/5000 dilution

    Lane 1: Human Phospholipase C beta 1 fragment recombinant protein at 0.01 µg

    Lane 2: Human Phospholipase C beta 3 fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 139 kDa

    Exposure time: 10s

  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Blocking/Dilution buffer: 5% NFDM/TBST.
    The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 17298601). Phospholipase C beta 1 selectively expresses in the brain which has been described in the literature (PMID: 2468162).

    All lanes: Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

    Lane 1: Mouse cerebellum lysate at 20 µg

    Lane 2: Rat cerebellum lysate at 20 µg

    Lane 3: Human fetal brain lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 3: Goat Anti-Rabbit IgG Peroxidase conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

    Exposure time: 3min

  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 & 4: 30 seconds; Lane 5,6,7 & 8:10 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 17298601). Phospholipase C beta 1 selectively expresses in the brain which has been described in the literature (PMID: 2468162).

    All lanes: Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Lane 7: Rat kidney lysate at 10 µg

    Lane 8: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

  • Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

    Lane 1: HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 10 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

    Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 139 kDa

    Observed band size: 150 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Mainly cytoplasm staining on neurons of the normal Human cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cells of the Mouse cerebellum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cell of the Rat cerebellum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunoprecipitation - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Phospholipase C beta 1 + Phospholipase C beta 3 were immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab184743 at 1/20 dilution.

    Western blot was performed from the immunoprecipitate using ab184743 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate, 10ug (Input).

    Lane 2: ab184743 IP in Mouse brain whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184743 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Observed band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Mouse liver. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Rat testis. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

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