AB220285

Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal

Name: Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (ab220285) | Abcam
Brand: Abcam Limited
SKU: AB220285
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Phospholipase C beta 2/PLCB2 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PLCB2 aa 1050 to C-terminus.

View Alternative Names

Phosphoinositide phospholipase C-beta-2, Phospholipase C-beta-2, PLC-beta-2, PLCB2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (AB220285)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (AB220285)

Immunofluorescent analysis of PFA fixed, triton X-100 permeabilized PC-3 cells labeling Phospholipase C beta 2/PLCB2 with ab220285 at 4 μg/mL (green).

Lab

Western blot - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (AB220285)

All lanes:

Western blot - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (ab220285) at 0.4 µg/mL

All lanes:

Human cell line HEL

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLCB2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q00722

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C beta 2 (PLCB2) also known as PLC-beta is an enzyme that plays an important role in the signaling pathways by helping in the conversion of phosphatidylinositol 45-bisphosphate into diacylglycerol and inositol trisphosphate. This reaction leads to the release of calcium ions from intracellular stores. PLCB2 has a molecular mass of approximately 134 kDa and it shows high expression in hematopoietic cells. Its presence is also detected in other tissues where specific signaling events take place.

Biological function summary

PLCB2 regulates important cellular functions by acting as an important mediator in signaling pathways that rely on G-protein-coupled receptors (GPCRs). It does not operate as part of a larger complex but interacts with G-proteins directly to propagate signals from the cell surface to the intracellular environment. This function establishes PLCB2 as an essential component in transmitting signals that control cell growth differentiation and more specialized activities in certain cell types.

Pathways

PLCB2's role is essential in both the phosphoinositide signaling and calcium signaling pathways. These pathways facilitate various physiological processes including muscle contraction secretion metabolism and others. In the phosphoinositide pathway PLCB2 cooperates closely with G-alpha-q proteins to propagate signals while in calcium signaling it helps modulate the release of calcium ions that activate many downstream proteins such as protein kinase C (PKC).

PLCB2 has been linked to certain conditions such as immune disorders and cardiac arrhythmias. In immune disorders dysregulated activity of PLCB2 can affect immune cell signaling while in cardiac arrhythmias abnormal calcium signaling due to PLCB2 misregulation impacts heart rhythm stability. Through these diseases proteins like PKC and G-alpha-q also play a significant role linking them to the dysfunction associated with altered PLCB2 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (PubMed : 1644792, PubMed : 9188725). In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity).

See full target information PLCB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 292:1950-1971 PubMed39871113

2025

Transcriptomic signatures and network-based methods uncover new senescent cell anti-apoptotic pathways and senolytics.

Applications

Unspecified application

Species

Unspecified reactive species

Samael Olascoaga,Mina Konigsberg,Jesús Espinal-Enríquez,Hugo Tovar,Félix Matadamas-Martínez,Jaime Pérez-Villanueva,Norma Edith López-Diazguerrero

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (AB220285)

Western blot - Anti-Phospholipase C beta 2/PLCB2 antibody - C-terminal (AB220285)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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