AB228794

Anti-Phospholipase C beta 3/PLCB3 antibody

Name: Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) | Abcam
Brand: Abcam Limited
SKU: AB228794
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Phospholipase C beta 3/PLCB3 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PLCB3.

View Alternative Names

Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3, PLC-beta-3, PLCB3

4 Images

Supplier Data

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

5% SDS-PAGE

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 139 kDa

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Supplier Data

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

5% SDS-PAGE

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 139 kDa

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Supplier Data

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

5% SDS-PAGE

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 139 kDa

true

Supplier Data

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

5% SDS-PAGE

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 7:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 139 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human PLCB3. The exact immunogen used to generate this antibody is proprietary information.

Q01970

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C beta 3 often called PLCB3 plays an important role in cellular signal transduction. This enzyme exists as part of the phospholipase C family enhances the conversion of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3). These molecules act as secondary messengers and are important to cellular responses. PLCB3 has a molecular mass of approximately 152 kDa. It exhibits significant expression in various tissues including the brain and heart among others where it regulates numerous physiological processes.

Biological function summary

The activation of PLCB3 influences intracellular calcium levels by producing IP3 contributing to calcium signaling pathways. As part of its biological role PLCB3 contributes to a dynamic signaling complex interacting with receptors like G-protein-coupled receptors (GPCRs) and affecting downstream signaling. This enzyme actively participates in regulating cellular processes such as proliferation and differentiation by mediating signal transduction pathways.

Pathways

PLCB3 integrates into the calcium signaling and phosphatidylinositol signaling pathways where it functions through interaction with G alpha q proteins. The activation of PLCB3 leads to the release of calcium ions from the endoplasmic reticulum a process important for many cellular functions. In these pathways PLCB3 shares interactions with other related proteins like protein kinase C (PKC) which further modulates cellular responses.

Dysregulation of PLCB3 activity has links to several pathophysiological conditions including cardiovascular diseases and cancer. Abnormal PLCB3 signaling correlates with irregular calcium homeostasis impacting cardiac functions and promoting oncogenic transformations. Interactions between PLCB3 and proteins such as GPCRs highlight their importance in developing therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 20966218, PubMed : 29122926, PubMed : 37991948, PubMed : 9188725). Key transducer of G protein-coupled receptor signaling : activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation (PubMed : 20966218, PubMed : 37991948). In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity).

See full target information PLCB3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animal : an international journal of animal bioscience 17:101017 PubMed37948891

2023

Comparative analysis of signalling pathways in tissue protein metabolism in efficient and non-efficient beef cattle: acute response to an identical single meal size.

Applications

Unspecified application

Species

Unspecified reactive species

P Guarnido-Lopez,I Ortigues-Marty,J David,S Polakof,G Cantalapiedra-Hijar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase C beta 3/PLCB3 antibody

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (AB228794)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise