AB41434

Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody

Name: Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (ab41434) | Abcam
Brand: Abcam Limited
SKU: AB41434

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(1 Publication)

Rabbit Polyclonal Phospholipase C gamma 1/PLC-gamma-1 phospho Y775 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PLCG1 phospho Y775.

View Alternative Names

PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1

1 Images

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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (AB41434)

Western blot analysis of Phospholipase C gamma 1/PLC-gamma-1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-Phospholipase C gamma 1/PLC-gamma-1. The blots were probed with anti- Phospholipase C gamma 1/PLC-gamma-1 (lanes 1 & 2) and anti-Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (ab41434)

Predicted band size: 148 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PLCG1 phospho Y775. The exact immunogen used to generate this antibody is proprietary information.

P19174

Specificity

Phospho-Tyr775-\Phospholipase C gamma 1/PLC-gamma-1 specific. Immunogen sequence has low homology to Phospholipase C gamma 2.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide, then affinity purified using phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr775) peptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 6 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.

Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed : 17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).

See full target information PLCG1 phospho Y775

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:3096848 PubMed40034562

2025

Taohong Siwu Decoction Combined With the LncRNA H19/miR-675-5p Axis Repairs Limb Ischemia-Reperfusion Injury Through the Regulation of the Wnt3a/Ca Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fuping Zhu,Hui Liu,Yinsheng Cao,Bing Dai,Hang Wu,Yutong Zhu,Wuping Li

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (AB41434)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise