AB4828

Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody

Name: Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab4828) | Abcam
Brand: Abcam Limited
SKU: AB4828
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal Phospholipase C gamma 1/PLC-gamma-1 phospho Y783 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PLCG1 phospho Y783.

View Alternative Names

PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (AB4828)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (AB4828)

ab5163 at a diluton of 1/1000 staining Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) in formalin fixed paraffin embedded sections of a) inflammed human gastric epithelium and b) normal human gastric epithelium by immunohistochemistry.

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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (AB4828)

Extracts prepared from NIH/3T3 (Mouse embryonic fibroblast cell line) cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 μg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show that PDGF is a strong inducer of Phospholipase C gamma 1/PLC-gamma-1 phosphorylation on Y783 in NIH3T3 cells. Extracts prepared from NIH-3T3 cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 μg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab4828)

Predicted band size: 148 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PLCG1 phospho Y783. The exact immunogen used to generate this antibody is proprietary information.

P19174

Specificity

Phosphorylation site-specific antibody selective for the phosphorylated form of the Phospholipase C gamma 1/PLC-gamma-1 containing a phosphate on tyrosine 783. The antibody has been shown to recognize the endogenous form of Phospholipase C gamma 1/PLC-gamma-1 when phosphorylated on tyrosine 783 in a variety of cell types following treatment by a broad range of extracellular stimuli. Applications include NIH 3T3 cells +/- PDGF. The antibody does not react with a site directed mutant (Y783F).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with nonphosphorylated Phospholipase C gamma 1/PLC-gamma-1 enzymes and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an Phospholipase C gamma 1/PLC-gamma-1 derived peptide that is phosphorylated at tyrosine 783.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.

Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed : 17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).

See full target information PLCG1 phospho Y783

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental biology 386:165-80 PubMed24269904

2013

Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization.

Applications

Unspecified application

Species

Xenopus laevis

Ryan C Bates,Colby P Fees,William L Holland,Courtney C Winger,Khulan Batbayar,Rachel Ancar,Todd Bergren,Douglas Petcoff,Bradley J Stith

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (AB4828)

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (AB4828)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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