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AB278707

Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4]

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Rabbit Recombinant Monoclonal Phospholipase C gamma 1/PLC-gamma-1 phospho Y783 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human PLCG1 phospho Y783.

View Alternative Names

PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1

4 Images
Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 μg/mL ab278707.

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)

ab278707 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278707.

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278707 at 0.1 ug/mL.

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278707)

Peptide blockage flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) treated with imatinib (red) treated with pervanadate (green) treated with PV + blocked with non-phospho- peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using ab278707 at 0.05 μg/mL.

  • 660 APC

    APC Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4]

  • 519 FITC

    FITC Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4]

  • 578 PE

    PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PLCG1Y783-C4

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human PLCG1 phospho Y783. The exact immunogen used to generate this antibody is proprietary information.

P19174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.88% PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.
Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed : 17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).
See full target information PLCG1 phospho Y783
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Product promise

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