Rabbit Polyclonal PLD1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Chinese hamster | Predicted | Predicted |
Pig | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Pig | Dilution info 1-5 µg/mL | Notes - |
Species Human | Dilution info 1-5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chinese hamster | Dilution info - | Notes - |
Select an associated product type
Function as phospholipase selective for phosphatidylcholine (PubMed:8530346, PubMed:9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1
Rabbit Polyclonal PLD1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus.
Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Phospholipase D (PLD) is an enzyme involved in the hydrolysis of phospholipids particularly phosphatidylcholine into phosphatidic acid and choline. This enzymatic reaction is important for signal transduction and membrane trafficking. In humans PLD primarily consists of two isoforms PLD1 and PLD2 each with a molecular mass of approximately 120-130 kDa. They are mainly expressed in the cellular membrane but also found in the cytosol and various intracellular organelles. These isoforms differ in their regulatory mechanisms and tissue distribution providing them specialized functions within the cell.
Phospholipase D plays roles in cellular processes such as cell proliferation differentiation and survival. The phospholipase D activity results in the production of phosphatidic acid a lipid mediator involved in vesicle trafficking and actin cytoskeleton remodeling. PLD functions within a protein complex with small GTP-binding proteins like ARF and Rho family members enhancing its catalytic activity and facilitating its involvement in complex cellular responses.
Phospholipase D participates in the mTOR signaling pathway and the PI3K-Akt pathway. These pathways are key to regulating cell growth metabolism and survival. PLD-generated phosphatidic acid acts as a signaling lipid that activates mTOR complex 1 a central component in the mTOR signaling pathway. Additionally phospholipase D interacts with proteins like PI4P5 kinase in the PI3K-Akt pathway linking it to phosphoinositide metabolism and indicating its integrative role in signaling networks.
Phospholipase D connects to cancer and neurodegenerative diseases. The overexpression and altered activity of PLD have been observed in several types of cancer particularly breast cancer where it is thought to contribute to tumor growth and metastasis. In Alzheimer's disease dysregulated PLD activity may be associated with abnormal phosphatidic acid levels impacting neural cell membrane integrity and signaling. In these contexts phospholipase D interacts with proteins such as amyloid precursor protein in Alzheimer's highlighting the broader implications of PLD in disease pathogenesis and potential therapeutic targeting.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
Formalin-fixed, paraffin-embedded human liver tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human glioma tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.
All lanes: Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL
All lanes: Recombinant human PLD1
All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 124 kDa
All lanes: Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL
All lanes: Pig small intestine tissue
All lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 124 kDa
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