AB233238

Anti-Phospholipase D antibody

Name: Anti-Phospholipase D antibody (ab233238) | Abcam
Brand: Abcam Limited
SKU: AB233238

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(2 Publications)

Rabbit Polyclonal PLD1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus.

View Alternative Names

Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human glioma tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human liver tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for PLD1 using ab233238 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Phospholipase D antibody (AB233238)

All lanes:

Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL

All lanes:

Pig small intestine tissue

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Phospholipase D antibody (AB233238)

All lanes:

Western blot - Anti-Phospholipase D antibody (ab233238) at 1 µg/mL

All lanes:

Recombinant human PLD1

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLD1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13393

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase D (PLD) is an enzyme involved in the hydrolysis of phospholipids particularly phosphatidylcholine into phosphatidic acid and choline. This enzymatic reaction is important for signal transduction and membrane trafficking. In humans PLD primarily consists of two isoforms PLD1 and PLD2 each with a molecular mass of approximately 120-130 kDa. They are mainly expressed in the cellular membrane but also found in the cytosol and various intracellular organelles. These isoforms differ in their regulatory mechanisms and tissue distribution providing them specialized functions within the cell.

Biological function summary

Phospholipase D plays roles in cellular processes such as cell proliferation differentiation and survival. The phospholipase D activity results in the production of phosphatidic acid a lipid mediator involved in vesicle trafficking and actin cytoskeleton remodeling. PLD functions within a protein complex with small GTP-binding proteins like ARF and Rho family members enhancing its catalytic activity and facilitating its involvement in complex cellular responses.

Pathways

Phospholipase D participates in the mTOR signaling pathway and the PI3K-Akt pathway. These pathways are key to regulating cell growth metabolism and survival. PLD-generated phosphatidic acid acts as a signaling lipid that activates mTOR complex 1 a central component in the mTOR signaling pathway. Additionally phospholipase D interacts with proteins like PI4P5 kinase in the PI3K-Akt pathway linking it to phosphoinositide metabolism and indicating its integrative role in signaling networks.

Phospholipase D connects to cancer and neurodegenerative diseases. The overexpression and altered activity of PLD have been observed in several types of cancer particularly breast cancer where it is thought to contribute to tumor growth and metastasis. In Alzheimer's disease dysregulated PLD activity may be associated with abnormal phosphatidic acid levels impacting neural cell membrane integrity and signaling. In these contexts phospholipase D interacts with proteins such as amyloid precursor protein in Alzheimer's highlighting the broader implications of PLD in disease pathogenesis and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Function as phospholipase selective for phosphatidylcholine (PubMed : 25936805, PubMed : 8530346, PubMed : 9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

See full target information PLD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:1562219 PubMed35836822

2022

Suppression of Long Noncoding RNA SNHG1 Inhibits the Development of Hypopharyngeal Squamous Cell Carcinoma via Increasing PARP6 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Xiao He,Bin Li,Jingjing Chen,Xuxia Tang

Journal of cellular physiology 235:6592-6604 PubMed32067228

2020

Promotion of cell autophagy and apoptosis in cervical cancer by inhibition of long noncoding RNA LINC00511 via transcription factor RXRA-regulated PLD1.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Shi,Mengran Liu,Yan Huang,Jing Zhang,Ling Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D antibody (AB233238)

Western blot - Anti-Phospholipase D antibody (AB233238)

Western blot - Anti-Phospholipase D antibody (AB233238)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise