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AB189191

Anti-Phospholipase D1 antibody

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(2 Publications)

Rabbit Polyclonal PLD1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLD1 aa 500-600.

View Alternative Names

Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D1 antibody (AB189191)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D1 antibody (AB189191)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue labeling PLD1 with ab189191 at 20 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PLD1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

Q13393

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase D1 (PLD1) is an enzyme belonging to the phospholipase family. It is also known by its gene name PLD1. This enzyme weighs approximately 120 kDa and plays an important mechanical role by hydrolyzing phosphatidylcholine to generate phosphatidic acid and choline. PLD1 is expressed majorly in the brain lungs and pancreas although it can be found in various other tissues as well.
Biological function summary

This enzyme is important in various cellular processes including membrane trafficking cell migration and signal transduction. PLD1 is not an isolated actor and works as part of signaling complexes contributing to cellular dynamics by generating lipid signaling molecules. It's associated with the regulation of mTOR signaling and interacts with other proteins such as protein kinase C (PKC) during various cellular processes.

Pathways

PLD1 plays significant roles in phosphoinositide signaling as well as the mTOR pathway. In the phosphoinositide signaling pathway PLD1 generates phosphatidic acid serving as a lipid signaling molecule which further activates mTOR. Proteins such as mTOR and S6K are related to PLD1's activity and help mediate cellular growth and proliferation activities.

Research links PLD1 to cancer and neurodegenerative diseases. Overexpression and hyperactivity of PLD1 are associated with the progression of certain cancers including breast cancer often in association with hyperactive mTOR signaling. Additionally PLD1's involvement in Alzheimer's disease has been studied given its role in signal transduction and membrane dynamics often correlating with the activity of tau proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Function as phospholipase selective for phosphatidylcholine (PubMed : 25936805, PubMed : 8530346, PubMed : 9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
See full target information PLD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7529 PubMed36477027

2022

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Applications

Unspecified application

Species

Unspecified reactive species

S Mouron,M J Bueno,A Lluch,L Manso,I Calvo,J Cortes,J A Garcia-Saenz,M Gil-Gil,N Martinez-Janez,J V Apala,E Caleiras,Pilar Ximénez-Embún,J Muñoz,L Gonzalez-Cortijo,R Murillo,R Sánchez-Bayona,J M Cejalvo,G Gómez-López,C Fustero-Torre,S Sabroso-Lasa,N Malats,M Martinez,A Moreno,D Megias,M Malumbres,R Colomer,M Quintela-Fandino

Frontiers in oncology 11:811635 PubMed35127525

2022

Aldolase A and Phospholipase D1 Synergistically Resist Alkylating Agents and Radiation in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chan Chang,Peter Mu-Hsin Chang,Chien-Hsiu Li,Ming-Hsien Chan,Yi-Jang Lee,Ming-Huang Chen,Michael Hsiao
View all publications
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