Rabbit Polyclonal PLD1 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PLD1 aa 500-600.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PLD1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q13393
Reactivity data
Select an application| IP | |
|---|---|
| Human | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Function as phospholipase selective for phosphatidylcholine (PubMed:8530346, PubMed:9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
Alternative names
Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1
Recommended products
Rabbit Polyclonal PLD1 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PLD1 aa 500-600.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PLD1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q13393
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phospholipase D1 (PLD1) is an enzyme belonging to the phospholipase family. It is also known by its gene name PLD1. This enzyme weighs approximately 120 kDa and plays an important mechanical role by hydrolyzing phosphatidylcholine to generate phosphatidic acid and choline. PLD1 is expressed majorly in the brain lungs and pancreas although it can be found in various other tissues as well.
- Biological function summary
This enzyme is important in various cellular processes including membrane trafficking cell migration and signal transduction. PLD1 is not an isolated actor and works as part of signaling complexes contributing to cellular dynamics by generating lipid signaling molecules. It's associated with the regulation of mTOR signaling and interacts with other proteins such as protein kinase C (PKC) during various cellular processes.
- Pathways
PLD1 plays significant roles in phosphoinositide signaling as well as the mTOR pathway. In the phosphoinositide signaling pathway PLD1 generates phosphatidic acid serving as a lipid signaling molecule which further activates mTOR. Proteins such as mTOR and S6K are related to PLD1's activity and help mediate cellular growth and proliferation activities.
- Associated diseases and disorders
Research links PLD1 to cancer and neurodegenerative diseases. Overexpression and hyperactivity of PLD1 are associated with the progression of certain cancers including breast cancer often in association with hyperactive mTOR signaling. Additionally PLD1's involvement in Alzheimer's disease has been studied given its role in signal transduction and membrane dynamics often correlating with the activity of tau proteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunoprecipitation - Anti-Phospholipase D1 antibody (ab240563)
PLD1 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) using ab240563 at 6 μg/mg lysate. Western blot was perfromed on the immunoprecipitate using ab240563 at 0.4 μg/ml.
Lane 1: ab240563 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-Phospholipase D1 antibody (ab240563)
Developed using the ECL technique.
Predicted band size: 124 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com