AB68150

Anti-Phospholipase D1 antibody [EP1506Y]

RabMAb
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(3 Publications)

Rabbit Monoclonal PLD1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Phospholipase D1, PLD 1, hPLD1, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1

2 Images

Lab

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (AB68150)

Blocking/Diluting buffer and concentration : 5% NFDM/TBS

Lanes 1 - 3:

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution

Lanes 1 - 3:

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/phospholipase-d1-antibody-ep1506y-bsa-and-azide-free-ab235997'>ab235997</a>) at 1/500 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) (RIPA) whole cell lysate 20ug

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) (RIPA) whole cell lysate 20ug

Lane 3:

C6 (Rat glial tumor glial cell) (RIPA) whole cell lysate 20ug

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 60s

Supplier Data

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (AB68150)

All lanes:

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 124 kDa

Observed band size: 120 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1506Y

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase D1 (PLD1) is an enzyme belonging to the phospholipase family. It is also known by its gene name PLD1. This enzyme weighs approximately 120 kDa and plays an important mechanical role by hydrolyzing phosphatidylcholine to generate phosphatidic acid and choline. PLD1 is expressed majorly in the brain lungs and pancreas although it can be found in various other tissues as well.

Biological function summary

This enzyme is important in various cellular processes including membrane trafficking cell migration and signal transduction. PLD1 is not an isolated actor and works as part of signaling complexes contributing to cellular dynamics by generating lipid signaling molecules. It's associated with the regulation of mTOR signaling and interacts with other proteins such as protein kinase C (PKC) during various cellular processes.

Pathways

PLD1 plays significant roles in phosphoinositide signaling as well as the mTOR pathway. In the phosphoinositide signaling pathway PLD1 generates phosphatidic acid serving as a lipid signaling molecule which further activates mTOR. Proteins such as mTOR and S6K are related to PLD1's activity and help mediate cellular growth and proliferation activities.

Research links PLD1 to cancer and neurodegenerative diseases. Overexpression and hyperactivity of PLD1 are associated with the progression of certain cancers including breast cancer often in association with hyperactive mTOR signaling. Additionally PLD1's involvement in Alzheimer's disease has been studied given its role in signal transduction and membrane dynamics often correlating with the activity of tau proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Function as phospholipase selective for phosphatidylcholine (PubMed : 25936805, PubMed : 8530346, PubMed : 9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

See full target information PLD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 19:43 PubMed33832505

2021

The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Helena M Stricker,Nadine Rommerswinkel,Silvia Keil,Sandina A Gnoth,Bernd Niggemann,Thomas Dittmar

Developmental cell 43:186-197.e7 PubMed29033361

2017

Binding of PLD2-Generated Phosphatidic Acid to KIF5B Promotes MT1-MMP Surface Trafficking and Lung Metastasis of Mouse Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqing Wang,Feng Zhang,Jingquan He,Ping Wu,Li Wei Rachel Tay,Ming Cai,Weiqi Nian,Yuanyuan Weng,Li Qin,Jeffrey T Chang,Laura B McIntire,Gilbert Di Paolo,Jianming Xu,Junmin Peng,Guangwei Du

Oncogene 31:3547-60 PubMed22105357

2011

PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D.

Applications

Unspecified application

Species

Unspecified reactive species

F J Sulzmaier,M K G Valmiki,D A Nelson,M J Caliva,D Geerts,M L Matter,E P White,J W Ramos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase D1 antibody [EP1506Y]

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (AB68150)

Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (AB68150)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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