JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242095

Anti-Phosphomevalonate kinase antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Phosphomevalonate kinase antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PMVK aa 100 to C-terminus.

View Alternative Names

PMKI, PMVK, Phosphomevalonate kinase, PMKase, hPMK

2 Images
Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (AB242095)
  • IP

Supplier Data

Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (AB242095)

Phosphomevalonate kinase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242095 at 8 μL/reaction. Western blot was performed from the immunoprecipitate using ab242095 at 1/1000 dilution.

Lane 1 : ab242095 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-Phosphomevalonate kinase antibody (ab242095)

Predicted band size: 21 kDa

false

Western blot - Anti-Phosphomevalonate kinase antibody (AB242095)
  • WB

Supplier Data

Western blot - Anti-Phosphomevalonate kinase antibody (AB242095)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-Phosphomevalonate kinase antibody (ab242095) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PMVK aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15126

style
left-column

Alternative Products

Primary Antibodies

AB65806

Anti-Phosphomevalonate kinase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphomevalonate kinase antibody (AB65806)

  • 0
  • 0
Primary Antibodies

AB199725

Anti-Phosphomevalonate kinase antibody [EPR15029]

RabMAb|Recombinant

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] (AB199725)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32352

Anti-HIV1 p24 antibody [P131]

RabMAb|Recombinant

Western blot - Anti-HIV1 p24 antibody [P131] (AB32352)

  • 0
  • 0
Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to Phosphomevalonate kinase immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomevalonate kinase (PMVK) is an enzyme involved in the mevalonate pathway a critical metabolic pathway that produces isoprenoids. Known also as mevalonate-5-phosphate kinase this enzyme catalyzes the ATP-dependent phosphorylation of mevalonate-5-phosphate to mevalonate-5-diphosphate. The molecular weight of PMVK is approximately 21 kDa. PMVK is expressed in various tissues with significant expression in the liver and adrenal glands reflecting its role in the biosynthesis of cholesterol and other isoprenoids.
Biological function summary

Phosphomevalonate kinase functions as an important regulatory enzyme in the synthesis of cholesterol and non-sterol isoprenoids. It does not operate as part of a larger protein complex but through its sole catalytic activity it impacts the production of downstream molecules essential for cell membrane integrity and steroid hormone production. The enzyme's activity can affect the availability of isoprenoid precursors linking metabolic flux through the mevalonate pathway to cellular growth and differentiation processes.

Pathways

Phosphomevalonate kinase plays a central role in the mevalonate pathway a vital metabolic pathway for cholesterol biosynthesis. It is responsible for converting mevalonate-5-phosphate to mevalonate-5-diphosphate a necessary step towards producing farnesyl pyrophosphate. This pathway is closely related to the biosynthesis of cholesterol and other essential isoprenoids. PMVK activity is associated with other enzymes in the pathway like HMG-CoA reductase a major control point in cholesterol biosynthesis.

Phosphomevalonate kinase's role in the mevalonate pathway relates to disorders involving cholesterol metabolism such as hypercholesterolemia and mevalonic aciduria. Mutations or misregulation of phosphomevalonate kinase can lead to altered cholesterol synthesis impacting cardiovascular health. Moreover PMVK may interact indirectly with proteins like LDL-receptor further linking its function to the regulation of cholesterol levels and associated diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.
See full target information PMVK
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com