AB65806

Anti-Phosphomevalonate kinase antibody

Name: Anti-Phosphomevalonate kinase antibody (ab65806) | Abcam
Brand: Abcam Limited
SKU: AB65806
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Phosphomevalonate kinase antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PMVK aa 100 to C-terminus.

View Alternative Names

PMKI, PMVK, Phosphomevalonate kinase, PMKase, hPMK

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphomevalonate kinase antibody (AB65806)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphomevalonate kinase antibody (AB65806)

Paraffin embedded rat skeletal muscle tissue stained for Phosphomevalonate kinase using ab65806 at 1 μg/ml in immunohistochemical analysis.

Unknown

Western blot - Anti-Phosphomevalonate kinase antibody (AB65806)

All lanes:

Western blot - Anti-Phosphomevalonate kinase antibody (ab65806) at 2 µg/mL

All lanes:

Rat cardiac muscle tissue lysate

Predicted band size: 21 kDa

Observed band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PMVK aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15126

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomevalonate kinase (PMVK) is an enzyme involved in the mevalonate pathway a critical metabolic pathway that produces isoprenoids. Known also as mevalonate-5-phosphate kinase this enzyme catalyzes the ATP-dependent phosphorylation of mevalonate-5-phosphate to mevalonate-5-diphosphate. The molecular weight of PMVK is approximately 21 kDa. PMVK is expressed in various tissues with significant expression in the liver and adrenal glands reflecting its role in the biosynthesis of cholesterol and other isoprenoids.

Biological function summary

Phosphomevalonate kinase functions as an important regulatory enzyme in the synthesis of cholesterol and non-sterol isoprenoids. It does not operate as part of a larger protein complex but through its sole catalytic activity it impacts the production of downstream molecules essential for cell membrane integrity and steroid hormone production. The enzyme's activity can affect the availability of isoprenoid precursors linking metabolic flux through the mevalonate pathway to cellular growth and differentiation processes.

Pathways

Phosphomevalonate kinase plays a central role in the mevalonate pathway a vital metabolic pathway for cholesterol biosynthesis. It is responsible for converting mevalonate-5-phosphate to mevalonate-5-diphosphate a necessary step towards producing farnesyl pyrophosphate. This pathway is closely related to the biosynthesis of cholesterol and other essential isoprenoids. PMVK activity is associated with other enzymes in the pathway like HMG-CoA reductase a major control point in cholesterol biosynthesis.

Phosphomevalonate kinase's role in the mevalonate pathway relates to disorders involving cholesterol metabolism such as hypercholesterolemia and mevalonic aciduria. Mutations or misregulation of phosphomevalonate kinase can lead to altered cholesterol synthesis impacting cardiovascular health. Moreover PMVK may interact indirectly with proteins like LDL-receptor further linking its function to the regulation of cholesterol levels and associated diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.

See full target information PMVK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 12:630097 PubMed33815288

2021

NT3/TrkC Pathway Modulates the Expression of UCP-1 and Adipocyte Size in Human and Rodent Adipose Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

María Bové,Fermi Monto,Paloma Guillem-Llobat,M Dolores Ivorra,M Antonia Noguera,Andrea Zambrano,M Salome Sirerol-Piquer,Ana Cristina Requena,Mauricio García-Alonso,Teresa Tejerina,José T Real,Isabel Fariñas,Pilar D'Ocon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphomevalonate kinase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphomevalonate kinase antibody (AB65806)

Western blot - Anti-Phosphomevalonate kinase antibody (AB65806)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise