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AB251290

Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Phosphomevalonate kinase antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PMKI, PMVK, Phosphomevalonate kinase, PMKase, hPMK

2 Images
Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (AB251290)
  • WB

Supplier Data

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (AB251290)

This data was developed using ab199725, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] (<a href='/en-us/products/primary-antibodies/phosphomevalonate-kinase-antibody-epr15029-ab199725'>ab199725</a>) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Lane 5:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

Exposure time: 3min

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (AB251290)
  • WB

Unknown

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (AB251290)

This data was developed using ab199725, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] (<a href='/en-us/products/primary-antibodies/phosphomevalonate-kinase-antibody-epr15029-ab199725'>ab199725</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

Exposure time: 1min

  • Unconjugated

    Anti-Phosphomevalonate kinase antibody [EPR15029]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15029

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251290 is the carrier-free version of ab199725.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomevalonate kinase (PMVK) is an enzyme involved in the mevalonate pathway a critical metabolic pathway that produces isoprenoids. Known also as mevalonate-5-phosphate kinase this enzyme catalyzes the ATP-dependent phosphorylation of mevalonate-5-phosphate to mevalonate-5-diphosphate. The molecular weight of PMVK is approximately 21 kDa. PMVK is expressed in various tissues with significant expression in the liver and adrenal glands reflecting its role in the biosynthesis of cholesterol and other isoprenoids.
Biological function summary

Phosphomevalonate kinase functions as an important regulatory enzyme in the synthesis of cholesterol and non-sterol isoprenoids. It does not operate as part of a larger protein complex but through its sole catalytic activity it impacts the production of downstream molecules essential for cell membrane integrity and steroid hormone production. The enzyme's activity can affect the availability of isoprenoid precursors linking metabolic flux through the mevalonate pathway to cellular growth and differentiation processes.

Pathways

Phosphomevalonate kinase plays a central role in the mevalonate pathway a vital metabolic pathway for cholesterol biosynthesis. It is responsible for converting mevalonate-5-phosphate to mevalonate-5-diphosphate a necessary step towards producing farnesyl pyrophosphate. This pathway is closely related to the biosynthesis of cholesterol and other essential isoprenoids. PMVK activity is associated with other enzymes in the pathway like HMG-CoA reductase a major control point in cholesterol biosynthesis.

Phosphomevalonate kinase's role in the mevalonate pathway relates to disorders involving cholesterol metabolism such as hypercholesterolemia and mevalonic aciduria. Mutations or misregulation of phosphomevalonate kinase can lead to altered cholesterol synthesis impacting cardiovascular health. Moreover PMVK may interact indirectly with proteins like LDL-receptor further linking its function to the regulation of cholesterol levels and associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.
See full target information PMVK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor
View all publications
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