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AB125864

Anti-Phosphoribosyl pyrophosphate amidotransferase antibody

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(4 Publications)

Rabbit Polyclonal Phosphoribosyl pyrophosphate amidotransferase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPAT aa 250-500.

View Alternative Names

GPAT, PPAT, Amidophosphoribosyltransferase, ATase, Glutamine phosphoribosylpyrophosphate amidotransferase

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (AB125864)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (AB125864)

Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling Phosphoribosyl pyrophosphate amidotransferase with ab125864 at 1/500 dilution. Costained with Hoechst 33342 (Bottom panel).

Western blot - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (AB125864)
  • WB

Unknown

Western blot - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (AB125864)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 57 kDa,70 kDa,83 kDa,85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPAT aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

Q06203

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoribosyl pyrophosphate amidotransferase also called PRPP amidotransferase is an enzyme that facilitates the conversion of phosphoribosyl pyrophosphate and glutamine into phosphoribosylamine in de novo purine synthesis. This enzyme has a molecular mass of approximately 59 kDa and is highly expressed in tissues with active nucleotide synthesis such as the liver and proliferating cells. Phosphoribosyl diphosphate amidotransferase serves a vital role in providing the building blocks for DNA and RNA synthesis.
Biological function summary

PRPP amidotransferase functions as an important regulator in the purine biosynthetic pathway. The enzyme does not function as part of a complex but instead acts independently to initiate the first committed step of purine nucleotide biosynthesis. It effectively sets the pace for the entire biosynthetic process responding to levels of its substrates and product feedback inhibition particularly by purine nucleotides.

Pathways

Purine biosynthesis involves the activity of PRPP amidotransferase which is central to both nucleotide biosynthesis and energy metabolism. This enzyme significantly cross-talks with the aminotransferase pathway to ensure proper cellular levels of nucleotides. It closely interacts with other enzymes such as adenylosuccinate synthetase to manage proper nucleoside and nucleotide production within cells.

PRPP amidotransferase imbalance is linked to various conditions such as gout and certain types of cancer. Dysregulation in enzyme activity can lead to increased purine production thereby contributing to hyperuricemia and gout. This enzyme is also connected to proteins like hypoxanthine-guanine phosphoribosyltransferase where imbalances in activity can exacerbate gout conditions or influence cancer cell proliferation. Understanding these connections aids in the development of therapeutic strategies targeting nucleotide synthesis pathways in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
See full target information PPAT
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 16:2428425 PubMed39567865

2024

Rotavirus rewires host cell metabolic pathways toward glutamine catabolism for effective virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Suvrotoa Mitra,Ratul Datta Chaudhuri,Rakesh Sarkar,Shreya Banerjee,Arpita Mukherjee,Ranjana Sharma,Animesh Gope,Kei Kitahara,Shin-Ichi Miyoshi,Mamta Chawla-Sarkar

International journal of molecular sciences 25: PubMed39273244

2024

Strategies to Maintain Redox Homeostasis in Yeast Cells with Impaired Fermentation-Dependent NADPH Generation.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Kwolek-Mirek,Roman Maslanka,Sabina Bednarska,Michał Przywara,Kornelia Kwolek,Renata Zadrag-Tecza

Cancer gene therapy 29:1878-1894 PubMed35840668

2022

MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Cai Zhang,Nicholas Skiados,Fareesa Aftab,Cerena Moreno,Luis Silva,Paul Joshua Anthony Corbilla,John M Asara,Aaron N Hata,Frank J Slack

Frontiers in oncology 10:541479 PubMed33102213

2020

Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Hu,Mingyang Bao,Jiacheng Huang,Lin Zhou,Shusen Zheng
View all publications
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