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AB181360

Anti-Phosphorylase B/PHKB antibody [EPR9035(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal Phosphorylase B/PHKB antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Phosphorylase b kinase regulatory subunit beta, Phosphorylase kinase subunit beta, PHKB

1 Images
Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] (AB181360)
  • WB

Supplier Data

Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] (AB181360)

All lanes:

Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] (ab181360) at 1/1000 dilution

Lane 1:

Human fetal muscle lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Lane 4:

Human fetal heart lysates at 10 µg

Predicted band size: 125 kDa

false

  • Carrier free

    Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9035(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Western blot - Anti-SPHK1 antibody [EPR24511-37] (AB302714)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S2P antibody (AB244279)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphorylase B also known as PHKB plays a significant mechanical role in glycogen metabolism. It exists as a catalytic subunit in the phosphorylase kinase complex essential for glycogen breakdown. This enzyme consists of two subunits phosphorylase a and b but phosphorylase B acts in an inactive state requiring conversion for activity. In humans PHKB has several isoforms and a mass around 125 kDa. This protein is mainly expressed in skeletal muscle and liver tissues highlighting its importance in energy regulation within these areas.
Biological function summary

PHKB facilitates the regulation of glycogenolysis by modulating phosphorylase kinase activity. As part of the larger phosphorylase kinase complex it activates when calcium ions bind allowing phosphorylase A to exert its function on glycogen phosphorylase. This activation results in the breakdown of glycogen into glucose-1-phosphate an important step in maintaining energy supply during muscle exertion and fasting. PHKB forms a multimeric complex with other subunits including alpha beta gamma and delta each contributing to its full enzymatic function.

Pathways

PHKB integrates within key metabolic pathways especially the glycogen metabolism and signaling pathways linked to energy homeostasis. It collaborates with phosphorylase A enzymes to convert glycogen stores to free glucose in response to hormonal and neuronal signals. This process involves interconnected protein interactions such as with the protein kinases PKA and calmodulin subunits to ensure precise regulation of glycogenolysis under variable physiological conditions.

Alterations in PHKB expression or function can lead to metabolic conditions. Glycogen storage disease type IXb a disorder characterized by muscle weakness and growth delay stems from mutations in the PHKB gene. This condition affects the ability to mobilize glycogen stores effectively resulting in restricted energy availability. PHKB's interaction with phosphorylase kinase subunits is vital in understanding its pathology connecting genetic mutations to broader metabolic dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
See full target information PHKB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 29:2647-2658 PubMed35378711

2022

Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yajing Wang,Fang Du,Zongyuan Xie,Junhao Lai,Yuanjie Li,Yongping Xu,Rui Tong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com