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AB250488

Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Phosphorylase B/PHKB antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Phosphorylase b kinase regulatory subunit beta, Phosphorylase kinase subunit beta, PHKB

1 Images
Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] - BSA and Azide free (AB250488)
  • WB

Supplier Data

Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] - BSA and Azide free (AB250488)

This data was developed using ab181360, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Phosphorylase B/PHKB antibody [EPR9035(2)] (<a href='/en-us/products/primary-antibodies/phosphorylase-b-phkb-antibody-epr90352-ab181360'>ab181360</a>) at 1/1000 dilution

Lane 1:

Human fetal muscle lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Lane 4:

Human fetal heart lysates at 10 µg

Predicted band size: 125 kDa

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  • Unconjugated

    Anti-Phosphorylase B/PHKB antibody [EPR9035(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9035(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250488 is the carrier-free version of ab181360.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphorylase B also known as PHKB plays a significant mechanical role in glycogen metabolism. It exists as a catalytic subunit in the phosphorylase kinase complex essential for glycogen breakdown. This enzyme consists of two subunits phosphorylase a and b but phosphorylase B acts in an inactive state requiring conversion for activity. In humans PHKB has several isoforms and a mass around 125 kDa. This protein is mainly expressed in skeletal muscle and liver tissues highlighting its importance in energy regulation within these areas.
Biological function summary

PHKB facilitates the regulation of glycogenolysis by modulating phosphorylase kinase activity. As part of the larger phosphorylase kinase complex it activates when calcium ions bind allowing phosphorylase A to exert its function on glycogen phosphorylase. This activation results in the breakdown of glycogen into glucose-1-phosphate an important step in maintaining energy supply during muscle exertion and fasting. PHKB forms a multimeric complex with other subunits including alpha beta gamma and delta each contributing to its full enzymatic function.

Pathways

PHKB integrates within key metabolic pathways especially the glycogen metabolism and signaling pathways linked to energy homeostasis. It collaborates with phosphorylase A enzymes to convert glycogen stores to free glucose in response to hormonal and neuronal signals. This process involves interconnected protein interactions such as with the protein kinases PKA and calmodulin subunits to ensure precise regulation of glycogenolysis under variable physiological conditions.

Alterations in PHKB expression or function can lead to metabolic conditions. Glycogen storage disease type IXb a disorder characterized by muscle weakness and growth delay stems from mutations in the PHKB gene. This condition affects the ability to mobilize glycogen stores effectively resulting in restricted energy availability. PHKB's interaction with phosphorylase kinase subunits is vital in understanding its pathology connecting genetic mutations to broader metabolic dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
See full target information PHKB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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