Rabbit Polyclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSAT1 aa 100 to C-terminus.
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Publications
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- Biology direct 18:732023p63 orchestrates serine and one carbon metabolism enzymes expression in head and neck cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela Cappello,Giulia Tosetti,Artem Smirnov,Carlo Ganini,Xue Yang,Yufang Shi,Ying Wang,Gerry Melino,Francesca Bernassola,Eleonora CandiPubMed 37946250
- Heliyon 9:e147992023Activating mutations in EGFR and PI3K promote ATF4 induction for NSCLC cell survival during amino acid deprivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMizuki Takahashi,Yuka Okamoto,Yu Kato,Hitomi Shirahama,Satomi Tsukahara,Yoshikazu Sugimoto,Akihiro TomidaPubMed 37025861
- Acta neuropathologica communications 9:162021Glioma cells require one-carbon metabolism to survive glutamine starvation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuhiro Tanaka et. al.PubMed 33468252
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PSAT1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y617
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.
Alternative names
PSA, PSAT1, Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT
Recommended products
Rabbit Polyclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSAT1 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PSAT1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y617
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphoserine Aminotransferase (PSAT1) is an enzyme that actively participates in amino acid metabolism. It is also known by alternate names like O-phospho-L-serine:2-oxoglutarate aminotransferase. With a molecular mass of approximately 39 kDa PSAT1 facilitates the conversion of 3-phosphohydroxypyruvate to phosphoserine. You can find its expression across various tissues including liver brain and kidney. This widespread presence suggests its essential role in cellular functions and metabolism.
- Biological function summary
PSAT1 plays an important role in the biosynthesis of the amino acid serine. It is part of a multi-step serine biosynthesis complex involving other enzymes like 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase. PSAT1 works in conjunction with these enzymes to ensure the proper synthesis of serine which is vital for nucleotide synthesis and cell proliferation. This function establishes PSAT1 as an important regulator of cellular growth and division.
- Pathways
PSAT1 is a significant component of the serine biosynthesis pathway which is linked to the folate cycle. This metabolic pathway is important for one-carbon metabolism affecting thymidine and purine synthesis. PSAT1 interacts with proteins such as 3-phosphoglycerate dehydrogenase highlighting its involvement in these interconnected metabolic processes. This connection indicates the critical position that PSAT1 holds in facilitating the production of biomolecules essential for cell survival and function.
- Associated diseases and disorders
PSAT1 has been associated with conditions such as Neu-Laxova syndrome and certain cancer types. Neu-Laxova syndrome linked to defects in serine biosynthesis involves abnormal neurological and physical development. Additionally PSAT1's role in cell proliferation pathways associates it with oncogenic processes where its altered expression can lead to tumor growth. Dysregulation of related proteins like phosphoserine phosphatase further ties PSAT1 to the pathogenesis of these conditions making it a target of interest in therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Phosphoserine Aminotransferase antibody (ab154055)
10% SDS PAGE
All lanes: Western blot - Anti-Phosphoserine Aminotransferase antibody (ab154055) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Western blot - Anti-Phosphoserine Aminotransferase antibody (ab154055)
10% SDS PAGE
All lanes: Western blot - Anti-Phosphoserine Aminotransferase antibody (ab154055) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 50 µg
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (ab154055)
Immunofluorescent analysis of methanol-fixed A549 cells, labeling Phosphoserine Aminotransferase with ab154055 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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