AB96136

Anti-Phosphoserine Aminotransferase antibody

Name: Anti-Phosphoserine Aminotransferase antibody (ab96136) | Abcam
Brand: Abcam Limited
SKU: AB96136
Rating: 5 (2 reviews)

(2 Reviews)

(19 Publications)

Rabbit Polyclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSAT1.

View Alternative Names

PSA, PSAT1, Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (AB96136)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (AB96136)

ab96136, at 1/200 staining paraformaldehyde-fixed A549 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).

Supplier Data

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136) at 1/1000 dilution

Lane 1:

A549 cell lysates at 30 µg

Lane 2:

H1299 cell lysates at 30 µg

Lane 3:

HCT116 cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136) at 1/1000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

PSAT1 shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PSAT1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y617

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoserine Aminotransferase (PSAT1) is an enzyme that actively participates in amino acid metabolism. It is also known by alternate names like O-phospho-L-serine:2-oxoglutarate aminotransferase. With a molecular mass of approximately 39 kDa PSAT1 facilitates the conversion of 3-phosphohydroxypyruvate to phosphoserine. You can find its expression across various tissues including liver brain and kidney. This widespread presence suggests its essential role in cellular functions and metabolism.

Biological function summary

PSAT1 plays an important role in the biosynthesis of the amino acid serine. It is part of a multi-step serine biosynthesis complex involving other enzymes like 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase. PSAT1 works in conjunction with these enzymes to ensure the proper synthesis of serine which is vital for nucleotide synthesis and cell proliferation. This function establishes PSAT1 as an important regulator of cellular growth and division.

Pathways

PSAT1 is a significant component of the serine biosynthesis pathway which is linked to the folate cycle. This metabolic pathway is important for one-carbon metabolism affecting thymidine and purine synthesis. PSAT1 interacts with proteins such as 3-phosphoglycerate dehydrogenase highlighting its involvement in these interconnected metabolic processes. This connection indicates the critical position that PSAT1 holds in facilitating the production of biomolecules essential for cell survival and function.

PSAT1 has been associated with conditions such as Neu-Laxova syndrome and certain cancer types. Neu-Laxova syndrome linked to defects in serine biosynthesis involves abnormal neurological and physical development. Additionally PSAT1's role in cell proliferation pathways associates it with oncogenic processes where its altered expression can lead to tumor growth. Dysregulation of related proteins like phosphoserine phosphatase further ties PSAT1 to the pathogenesis of these conditions making it a target of interest in therapeutic research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.

See full target information PSAT1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:340 PubMed38098117

2023

AMPK-HIF-1α signaling enhances glucose-derived de novo serine biosynthesis to promote glioblastoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Jin Yun,Min Li,Dong Guo,So Mi Jeon,Su Hwan Park,Je Sun Lim,Su Bin Lee,Rui Liu,Linyong Du,Seok-Ho Kim,Tae Hwan Shin,Seong-Il Eyun,Yun-Yong Park,Zhimin Lu,Jong-Ho Lee

Journal of translational medicine 21:70 PubMed36732787

2023

PSAT1 positively regulates the osteogenic lineage differentiation of periodontal ligament stem cells through the ATF4/PSAT1/Akt/GSK3β/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Linglu Jia,Dongfang Li,Ya-Nan Wang,Dongjiao Zhang,Xin Xu

Journal of immunology (Baltimore, Md. : 1950) 209:1189-1199 PubMed36002234

2022

A Rapid Translational Immune Response Program in CD8 Memory T Lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Darin Salloum,Kamini Singh,Natalie R Davidson,Linlin Cao,David Kuo,Viraj R Sanghvi,Man Jiang,Maria Tello Lafoz,Agnes Viale,Gunnar Ratsch,Hans-Guido Wendel

Nature communications 12:6176 PubMed34702840

2021

The impact of physiological metabolite levels on serine uptake, synthesis and utilization in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Hennequart,Christiaan F Labuschagne,Mylène Tajan,Steven E Pilley,Eric C Cheung,Nathalie M Legrave,Paul C Driscoll,Karen H Vousden

Cell metabolism 33:1793-1807.e9 PubMed34358431

2021

Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4 endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Guillermo Turiel,Raphaela Ardicoglu,Moheb Ghobrial,Evi Masschelein,Tea Kocijan,Jing Zhang,Ge Tan,Gillian Fitzgerald,Tatiane Gorski,Abdiel Alvarado-Diaz,Paola Gilardoni,Christopher M Adams,Bart Ghesquière,Katrien De Bock

Human molecular genetics 30:2012-2026 PubMed34169315

2021

Coordinated pyruvate kinase activity is crucial for metabolic adaptation and cell survival during mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshan Zhou,Flora Mikaeloff,Sophie Curbo,Qian Zhao,Raoul Kuiper,Ákos Végvári,Ujjwal Neogi,Anna Karlsson

Nature communications 12:366 PubMed33446657

2021

Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Mylène Tajan,Marc Hennequart,Eric C Cheung,Fabio Zani,Andreas K Hock,Nathalie Legrave,Oliver D K Maddocks,Rachel A Ridgway,Dimitris Athineos,Alejandro Suárez-Bonnet,Robert L Ludwig,Laura Novellasdemunt,Nikolaos Angelis,Vivian S W Li,Georgios Vlachogiannis,Nicola Valeri,Nello Mainolfi,Vipin Suri,Adam Friedman,Mark Manfredi,Karen Blyth,Owen J Sansom,Karen H Vousden

Aging 13:1212-1235 PubMed33291071

2020

Therapeutic potential of targeting through dual regulation of two candidate prognostic biomarkers and in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Tang,Lingli Luo,Yukun Li,Hailong Wu,Qing Hu,Haiyan Yue,Xiao He,Juan Zou,Shaoxiong Min

Scientific reports 10:16769 PubMed33028928

2020

The effects of fructose and metabolic inhibition on hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany Dewdney,Mohammed Alanazy,Rhys Gillman,Sarah Walker,Miriam Wankell,Liang Qiao,Jacob George,Alexandra Roberts,Lionel Hebbard

Annals of clinical and laboratory science 50:512-518 PubMed32826249

2020

PSAT1 Upregulation Contributes to Cell Growth and Cisplatin Resistance in Cervical Cancer Cells via Regulating PI3K/AKT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxu Duan,Xiaomei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Phosphoserine Aminotransferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Western blot - Anti-Phosphoserine Aminotransferase antibody (AB96136)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise