Rabbit Polyclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSAT1.
Images
Publications
Filter by
- Journal of experimental & clinical cancer research : CR 42:3402023AMPK-HIF-1α signaling enhances glucose-derived de novo serine biosynthesis to promote glioblastoma growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye Jin Yun,Min Li,Dong Guo,So Mi Jeon,Su Hwan Park,Je Sun Lim,Su Bin Lee,Rui Liu,Linyong Du,Seok-Ho Kim,Tae Hwan Shin,Seong-Il Eyun,Yun-Yong Park,Zhimin Lu,Jong-Ho LeePubMed 38098117
- Journal of translational medicine 21:702023PSAT1 positively regulates the osteogenic lineage differentiation of periodontal ligament stem cells through the ATF4/PSAT1/Akt/GSK3β/β-catenin axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinglu Jia,Dongfang Li,Ya-Nan Wang,Dongjiao Zhang,Xin XuPubMed 36732787
- Journal of immunology (Baltimore, Md. : 1950) 209:1189-11992022A Rapid Translational Immune Response Program in CD8 Memory T Lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarin Salloum,Kamini Singh,Natalie R Davidson,Linlin Cao,David Kuo,Viraj R Sanghvi,Man Jiang,Maria Tello Lafoz,Agnes Viale,Gunnar Ratsch,Hans-Guido WendelPubMed 36002234
- Nature communications 12:61762021The impact of physiological metabolite levels on serine uptake, synthesis and utilization in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc Hennequart,Christiaan F Labuschagne,Mylène Tajan,Steven E Pilley,Eric C Cheung,Nathalie M Legrave,Paul C Driscoll,Karen H VousdenPubMed 34702840
- Cell metabolism 33:1793-1807.e92021Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4 endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Fan,Guillermo Turiel,Raphaela Ardicoglu,Moheb Ghobrial,Evi Masschelein,Tea Kocijan,Jing Zhang,Ge Tan,Gillian Fitzgerald,Tatiane Gorski,Abdiel Alvarado-Diaz,Paola Gilardoni,Christopher M Adams,Bart Ghesquière,Katrien De BockPubMed 34358431
- Human molecular genetics 30:2012-20262021Coordinated pyruvate kinase activity is crucial for metabolic adaptation and cell survival during mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoshan Zhou,Flora Mikaeloff,Sophie Curbo,Qian Zhao,Raoul Kuiper,Ákos Végvári,Ujjwal Neogi,Anna KarlssonPubMed 34169315
- Nature communications 12:3662021Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMylène Tajan et. al.PubMed 33446657
- Aging 13:1212-12352020Therapeutic potential of targeting through dual regulation of two candidate prognostic biomarkers and in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojun Tang et. al.PubMed 33291071
- Scientific reports 10:167692020The effects of fructose and metabolic inhibition on hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany Dewdney et. al.PubMed 33028928
- Annals of clinical and laboratory science 50:512-5182020PSAT1 Upregulation Contributes to Cell Growth and Cisplatin Resistance in Cervical Cancer Cells via Regulating PI3K/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxu Duan,Xiaomei LiuPubMed 32826249
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PSAT1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y617
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.
Alternative names
PSA, PSAT1, Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT
Recommended products
Rabbit Polyclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSAT1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PSAT1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y617
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphoserine Aminotransferase (PSAT1) is an enzyme that actively participates in amino acid metabolism. It is also known by alternate names like O-phospho-L-serine:2-oxoglutarate aminotransferase. With a molecular mass of approximately 39 kDa PSAT1 facilitates the conversion of 3-phosphohydroxypyruvate to phosphoserine. You can find its expression across various tissues including liver brain and kidney. This widespread presence suggests its essential role in cellular functions and metabolism.
- Biological function summary
PSAT1 plays an important role in the biosynthesis of the amino acid serine. It is part of a multi-step serine biosynthesis complex involving other enzymes like 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase. PSAT1 works in conjunction with these enzymes to ensure the proper synthesis of serine which is vital for nucleotide synthesis and cell proliferation. This function establishes PSAT1 as an important regulator of cellular growth and division.
- Pathways
PSAT1 is a significant component of the serine biosynthesis pathway which is linked to the folate cycle. This metabolic pathway is important for one-carbon metabolism affecting thymidine and purine synthesis. PSAT1 interacts with proteins such as 3-phosphoglycerate dehydrogenase highlighting its involvement in these interconnected metabolic processes. This connection indicates the critical position that PSAT1 holds in facilitating the production of biomolecules essential for cell survival and function.
- Associated diseases and disorders
PSAT1 has been associated with conditions such as Neu-Laxova syndrome and certain cancer types. Neu-Laxova syndrome linked to defects in serine biosynthesis involves abnormal neurological and physical development. Additionally PSAT1's role in cell proliferation pathways associates it with oncogenic processes where its altered expression can lead to tumor growth. Dysregulation of related proteins like phosphoserine phosphatase further ties PSAT1 to the pathogenesis of these conditions making it a target of interest in therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136)
10% SDS PAGE
All lanes: Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (ab96136)
ab96136, at 1/200 staining paraformaldehyde-fixed A549 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com