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Rabbit Recombinant Monoclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512), expandable thumbnail
  • Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512), expandable thumbnail
  • Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512), expandable thumbnail
  • Immunoprecipitation - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.

Alternative names

PSA, PSAT1, Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT

Recommended products

Rabbit Recombinant Monoclonal Phosphoserine Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26994-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Phosphoserine Aminotransferase (PSAT1) is an enzyme that actively participates in amino acid metabolism. It is also known by alternate names like O-phospho-L-serine:2-oxoglutarate aminotransferase. With a molecular mass of approximately 39 kDa PSAT1 facilitates the conversion of 3-phosphohydroxypyruvate to phosphoserine. You can find its expression across various tissues including liver brain and kidney. This widespread presence suggests its essential role in cellular functions and metabolism.

Biological function summary

PSAT1 plays an important role in the biosynthesis of the amino acid serine. It is part of a multi-step serine biosynthesis complex involving other enzymes like 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase. PSAT1 works in conjunction with these enzymes to ensure the proper synthesis of serine which is vital for nucleotide synthesis and cell proliferation. This function establishes PSAT1 as an important regulator of cellular growth and division.

Pathways

PSAT1 is a significant component of the serine biosynthesis pathway which is linked to the folate cycle. This metabolic pathway is important for one-carbon metabolism affecting thymidine and purine synthesis. PSAT1 interacts with proteins such as 3-phosphoglycerate dehydrogenase highlighting its involvement in these interconnected metabolic processes. This connection indicates the critical position that PSAT1 holds in facilitating the production of biomolecules essential for cell survival and function.

Associated diseases and disorders

PSAT1 has been associated with conditions such as Neu-Laxova syndrome and certain cancer types. Neu-Laxova syndrome linked to defects in serine biosynthesis involves abnormal neurological and physical development. Additionally PSAT1's role in cell proliferation pathways associates it with oncogenic processes where its altered expression can lead to tumor growth. Dysregulation of related proteins like phosphoserine phosphatase further ties PSAT1 to the pathogenesis of these conditions making it a target of interest in therapeutic research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Phosphoserine Aminotransferase with ab308512 at 1/50 (9.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HepG2 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512), expandable thumbnail

    Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: human skeletal muscle (PMID: 12633500).

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate, at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

    Exposure time: 15s

  • Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512), expandable thumbnail

    Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 3: 293T transfected with siRNA specifically targeti Phosphoserine Aminotransferase whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

    Exposure time: 3.25s

  • Flow Cytometry (Intracellular) - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Phosphoserine Aminotransferase with ab308512 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512), expandable thumbnail

    Immunoprecipitation - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512)

    Phosphoserine Aminotransferase was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab308512 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308512 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 2: abAB308512 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308512 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds

    All lanes: Immunoprecipitation - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (ab308512) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 40 kDa

    Exposure time: 5s

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Product protocols

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