Anti-Phosphoserine antibody is a rabbit polyclonal antibody that is used to detect Phosphoserine in western blot. Suitable for chemical samples.
- Recognize proteins phosphorylated on serine residues
- Does not cross-react with phosphotyrosine
- Cited in over 150 publications
- Trusted since 2002
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- Life science alliance 7:2023FAT10 is phosphorylated by IKKβ to inhibit the antiviral type-I interferon response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKritika Saxena,Nicola Domenico Roverato,Melody Reithmann,Mei Min Mah,Richard Schregle,Gunter Schmidtke,Ivan Silbern,Henning Urlaub,Annette AichemPubMed 37940187
- The EMBO journal 42:e1132582023NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhao,Jiaojiao Zhang,Hong Lei,Yuanyuan Meng,Hongcheng Cheng,Yanping Zhao,Guangfeng Geng,Chenglong Mu,Linbo Chen,Qiangqiang Liu,Qian Luo,Chuanmei Zhang,Yijia Long,Jingyi Su,Yinhao Wang,Zhuoya Li,Jiaxing Sun,Guo Chen,Yanjun Li,Xudong Liao,Yingli Shang,Gang Hu,Quan Chen,Yushan ZhuPubMed 37409632
- Oncogene 42:2439-24552023Phosphorylated PTTG1 switches its subcellular distribution and promotes β-catenin stabilization and subsequent transcription activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuewen Zhang,Nianping Wu,Huili Huang,Shi Li,Shicheng Liu,Rui Zhang,Yuan Huang,Hao Lyu,Shuai Xiao,Declan William Ali,Marek Michalak,Xing-Zhen Chen,Cefan Zhou,Jingfeng TangPubMed 37400529
- International journal of molecular sciences 24:2023The Phosphoproteome of the Rd1 Mouse Retina, a Model of Inherited Photoreceptor Degeneration, Changes after Protein Kinase G Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaming Zhou,Charlotte Welinder,Per EkströmPubMed 37372984
- Molecular and cellular biology 43:223-2402023EPRS1 Controls the TGF- Signaling Pathway via Interaction with TβRI in Hepatic Stellate Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIna Yoon,Ji Ae Song,Ji Hun Suh,Sulhee Kim,Jonghyeon Son,Jong Hyun Kim,Song Yee Jang,Kwang Yeon Hwang,Myung Hee Kim,Sunghoon KimPubMed 37154023
- Frontiers in microbiology 14:11125842023Phosphorylation status of a conserved residue in the adenylate cyclase of is involved in regulating photomorphogenesis, circadian rhythm, and pathogenicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfei Cai,Xue Chen,Peixuan Li,Weiheng Ren,Qiang Zhang,Yiwen Wang,Yina Jiang,Pinkuan Zhu,Hideyoshi Toyoda,Ling XuPubMed 36876105
- Journal of Cancer 14:200-2182023Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jia,Hui-Yan Li,Jue Wang,Xing Chen,Lu Lou,Yan-Yan Wei,Ying Wang,Shi-Jing MoPubMed 36741265
- Journal of medical virology 95:e284002022Phosphorylation of enteroviral 2A at Ser/Thr125 benefits its proteolytic activity and viral pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuya Wang,Wenjia Zou,Yan Niu,Sanyuan Wang,Bangtao Chen,Rui Xiong,Peng Zhang,Zhijun Luo,Yong Wu,Changfa Fan,Zhaohua Zhong,Ping Xu,Yihong PengPubMed 36511115
- Development (Cambridge, England) 149:2022Chemokine signaling synchronizes angioblast proliferation and differentiation during pharyngeal arch artery vasculogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu,Mingming Zhang,Haojian Dong,Jingwen Liu,Aihua Mao,Guozhu Ning,Yu Cao,Yiyue Zhang,Qiang WangPubMed 36468454
- Nature communications 13:67812022CK2-mediated phosphorylation of SUZ12 promotes PRC2 function by stabilizing enzyme active site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihu Gong,Xiuli Liu,Lianying Jiao,Xin Yang,Andrew Lemoff,Xin LiuPubMed 36351927
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.9% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to Phosphoserine.
Reactivity data
Select an application| WB | |
|---|---|
| Chemical | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. |
Associated Products
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3 products for Alternative Product
Target data
Alternative names
PS, pSER, phospho-Ser
Recommended products
Anti-Phosphoserine antibody is a rabbit polyclonal antibody that is used to detect Phosphoserine in western blot. Suitable for chemical samples.
- Recognize proteins phosphorylated on serine residues
- Does not cross-react with phosphotyrosine
- Cited in over 150 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.9% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to Phosphoserine.
- Purification technique
- Affinity purification Immunogen
- Specificity
Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA.Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Phosphoserine antibody (ab9332) is a rabbit polyclonal antibody and is validated for use in WB.
Anti-Phosphoserine antibody (ab9332) specifically detects Phosphoserine (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Phosphoserine antibody (ab9332) has high sensitivity and specificity.
Anti-Phosphoserine antibody (ab9332) has been cited over 156 times in peer reviewed journals and is trusted by the scientific community.
Anti-Phosphoserine antibody (ab9332) has 9 independent reviews from customers.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
This image is courtesy of a customer review submitted by Karin Birkenkamp-DemtroederWestern blot - Anti-Phosphoserine antibody (ab9332)
Blocking Step: 10% Milk for 1 hour at room temperature
All lanes: Western blot - Anti-Phosphoserine antibody (ab9332) at 3 µg/mL
All lanes: Whole cell lysate of monkey COS7 cells at 25 µg
SecondaryAll lanes: An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Western blot - Anti-Phosphoserine antibody (ab9332)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies.
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.Lane 1: Western blot - Biotin Anti-Phosphoserine antibody (Biotin Anti-Phosphoserine antibody ab9335)
Lane 1: Western blot - Anti-Phosphoserine antibody (ab9332)
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