Anti-Phosphoserine antibody

• WB

AbReview19643****

Western blot - Anti-Phosphoserine antibody (AB9332)

Blocking Step : 10% Milk for 1 hour at room temperature

All lanes:

Western blot - Anti-Phosphoserine antibody (ab9332) at 3 µg/mL

All lanes:

Whole cell lysate of monkey COS7 cells at 25 µg

Secondary

All lanes:

An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution

false

This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder

• WB

Unknown

Western blot - Anti-Phosphoserine antibody (AB9332)

Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies

The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.

The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.

Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.

Lane 1:

Western blot - Biotin Anti-Phosphoserine antibody (<a href='/en-us/products/primary-antibodies/biotin-phosphoserine-antibody-ab9335'>ab9335</a>)

Lane 1:

Western blot - Anti-Phosphoserine antibody (ab9332)

false