AB9337

Anti-Phosphothreonine antibody

Name: Anti-Phosphothreonine antibody. Rabbit polyclonal (ab9337) | Abcam
Brand: Abcam Limited
SKU: AB9337
Price: 565 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(47 Publications)

Rabbit Polyclonal Phosphothreonine antibody. Suitable for IP, ELISA, WB, IHC-Fr and reacts with Modified Amino Acid, Chemical, Mouse samples. Cited in 47 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphothreonine.

2 Images

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Western blot - Anti-Phosphothreonine antibody (AB9337)

Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B) Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

Lane 1:

Western blot - HRP Anti-Phosphothreonine antibody (<a href='/en-us/products/primary-antibodies/hrp-phosphothreonine-antibody-ab9338'>ab9338</a>)

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Western blot - Anti-Phosphothreonine antibody (ab9337)

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Western blot - Biotin Anti-Phosphothreonine antibody (<a href='/en-us/products/primary-antibodies/biotin-phosphothreonine-antibody-ab9340'>ab9340</a>)

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ELISA - Anti-Phosphothreonine antibody (AB9337)

ELISA

Unknown

ELISA - Anti-Phosphothreonine antibody (AB9337)

Antibody Capture ELISA
Label : immobilized antigen

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-Fr, ELISA, WB, IP

applications

Specificity

Recognize proteins phosphorylated on threonine residues. Not cross-reacted with phosphotyrosine.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chemical": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.5 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Modified Amino Acid": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use at a concentration of 10 µg/mg. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphothreonine often called phospho-threonine refers to a modified amino acid where a phosphate group attaches to threonine. It usually forms during protein post-translational modifications. This modification commonly occurs in eukaryotic cells and plays a significant role in regulating protein function. The mass of phosphothreonine itself is approximately 181 Da. Phosphothreonine residues are found in various proteins where they serve as important components for controlling multiple cellular processes.

Biological function summary

Phosphorylation of threonine residues influences protein activity localization and interactions. These phosphorylated threonine residues often exist as part of a larger protein complex influencing the dynamics of protein interactions. In cellular signal transduction they act as key regulatory switches. The modification is a reversible process which allows dynamic control over protein functionality in response to cellular signals.

Pathways

Signaling cascades often rely on phosphorylation events involving threonine which includes pathways like MAPK and PI3K/AKT. These pathways are essential for cellular responses to external stimuli and involve several other proteins such as RAS and AKT themselves. Through such pathways the phosphorylation state of threonine residues affects decisions about cell survival proliferation or differentiation.

Dysregulation of threonine phosphorylation links to conditions like cancer and diabetes. Abnormal phosphorylation patterns can lead to unregulated cell growth and survival common in tumorigenesis. Proteins such as p53 and insulin receptor substrates interact with phosphorylated threonine residues in these contexts. Investigating these interactions provides insights into the pathological mechanisms and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Phosphothreonine

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Publications (47)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:31802 PubMed40877392

2025

Regulation of divergent epithelial-to-mesenchymal transition responses via the CDK4/6-USP51 pathway through ZEB1 protein stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Sakamoto,Masataka Takahashi,Hajime Nishimura,Kazuhide Watanabe,Harukazu Suzuki

Nature 646:198-207 PubMed40739344

2025

Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Paul,Derek L Bolhuis,Hualong Yan,Sudipto Das,Xia Xu,Christina C Abbate,Lisa M M Jenkins,Michael J Emanuele,Thorkell Andresson,Jing Huang,John G Albeck,Nicholas G Brown,Steven D Cappell

Acta pharmaceutica Sinica. B 14:3049-3067 PubMed39027246

2024

PIM1-HDAC2 axis modulates intestinal homeostasis through epigenetic modification.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Yang,Yawen Xiao,Ningning Zhao,Geng Pei,Yan Sun,Xinyu Sun,Kaiyuan Yu,Chunhui Miao,Ran Liu,Junqiang Lv,Hongyu Chu,Lu Zhou,Bangmao Wang,Zhi Yao,Quan Wang

Cell death & disease 15:395 PubMed38839744

2024

Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Liu,Ke Shao,Wendong Yang,Qi Shen,Mengru Lu,Zhiying Shao,Sufang Chu,Yuming Wang,Xuehao Wang,Xiaofeng Chen,Jin Bai,Xiaofeng Wu

The EMBO journal 42:e113258 PubMed37409632

2023

NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Zhao,Jiaojiao Zhang,Hong Lei,Yuanyuan Meng,Hongcheng Cheng,Yanping Zhao,Guangfeng Geng,Chenglong Mu,Linbo Chen,Qiangqiang Liu,Qian Luo,Chuanmei Zhang,Yijia Long,Jingyi Su,Yinhao Wang,Zhuoya Li,Jiaxing Sun,Guo Chen,Yanjun Li,Xudong Liao,Yingli Shang,Gang Hu,Quan Chen,Yushan Zhu

Pflugers Archiv : European journal of physiology 475:583-593 PubMed36917272

2023

Calcineurin-dependent regulation of gap junction conductance and connexin phosphorylation in guinea pig left atrium.

Applications

Unspecified application

Species

Unspecified reactive species

R I Jabr,S C Salvage,F S Hatch,C H Fry

Journal of medical virology 95:e28400 PubMed36511115

2022

Phosphorylation of enteroviral 2A at Ser/Thr125 benefits its proteolytic activity and viral pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Wang,Wenjia Zou,Yan Niu,Sanyuan Wang,Bangtao Chen,Rui Xiong,Peng Zhang,Zhijun Luo,Yong Wu,Changfa Fan,Zhaohua Zhong,Ping Xu,Yihong Peng

Development (Cambridge, England) 149: PubMed36468454

2022

Chemokine signaling synchronizes angioblast proliferation and differentiation during pharyngeal arch artery vasculogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Mingming Zhang,Haojian Dong,Jingwen Liu,Aihua Mao,Guozhu Ning,Yu Cao,Yiyue Zhang,Qiang Wang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22468 PubMed35913801

2022

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chujie Ding,Yu Hong,Yuan Che,Tianyu He,Yun Wang,Shule Zhang,Jiawei Wu,Wanfeng Xu,Jingyi Hou,Haiping Hao,Lijuan Cao

Cancer science 113:2753-2762 PubMed35722967

2022

Hippo pathway monomerizes STAT3 to regulate prostate cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfeng Tang,Jing Fang,Weiqi Lai,Yu Hu,Chengwan Liu,Xiaobo Hu,Caiyong Song,Tianmu Cheng,Rui Liu,Xiaoke Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphothreonine antibody

Western blot - Anti-Phosphothreonine antibody (AB9337)

ELISA - Anti-Phosphothreonine antibody (AB9337)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (47)

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